We introduce an in situ mouse model of lung cancer that utilizes direct injection of cancer cells into lung tissue, allowing for many potential studies.

The specific experimental process is as follows:

1. Prepare cancer cells for injection;

2. Anesthesia of C57 mice;

3. Iodine disinfection;

4. Cut a skin incision of approximately 1 cm horizontally at the lower edge of the left scapula, about 0.5 cm away;

5. Separate and widen the incision area to ensure that all layers are cut open and the ribs are visible, revealing the light pink structure of the lungs beneath the chest cavity;

Slowly inject 20ul of cancer cells between ribs 5 and 6, and the lungs of mice are widest and thickest in this area;

7. Close the incision.

Ilebo Biotechnology (Shanghai) Co., Ltd. has rich research experience, complete systematic management capabilities, and a scientific research service system. At present, we have built nearly 1000 square meters of experimental platforms for cells, molecules, pathology, etc., equipped with instruments and equipment, cell banks, sample preservation banks, etc. The animal experimental center covers an area of nearly 1800 square meters and can accommodate both clean and SPF grade animals. The core experimental technology team mainly comes from key universities and research institutions such as Fudan University and Shanghai Jiao Tong University, and can provide technical support for clients in research scheme planning, project implementation, and in-depth project analysis. The company is currently involved in research projects covering various fields such as cardiovascular and cerebrovascular diseases, tumors, endocrinology, reproduction, immunology, and neurology.