The effect of thyroxine on the proliferation of osteoblasts, alkaline phosphatase activity, and osteocalcin content in rats

To explore thyroid hormone (T)₄）The effects of enzymatic digestion on the proliferation, alkaline phosphatase (ALP) activity, and osteocalcin (OC) content of primary neonatal SD rat skull osteoblasts cultured in vitro were studied. The osteoblasts were exposed to a final concentration of 0 (control) and 10^-9、 ten^-8, 10^-7mol/L T₄Cultivate in the culture medium for 24, 48, and 72 hours, and observe the proliferation of osteoblasts; After the end of the poisoning, the ALP activity and OC content of the cells were detected. The results showed that compared with the control group, 10^-8, 10^-7mol/L T₄The survival rate of osteoblasts was higher after 24, 48, and 72 hours of exposure, and the difference was statistically significant (P<0.05); And 10^-9mol/L T₄There was no significant change in the survival rate of exposed bone cells. And with T₄The increase in exposure concentration and the prolongation of exposure time lead to an upward trend in the survival rate of osteoblasts. Compared with the control group, 10^-8, 10^-7mol/L T₄The ALP activity and OC content of exposed bone cells were both high, and the differences were statistically significant (P<0.05); And 10^-9mol/L T₄There was no significant change in ALP activity and OC content of exposed bone cells. And with T₄With the increase of exposure concentration, the ALP activity and OC content of osteoblasts showed an upward trend, indicating that T₄Has a certain bone promoting effect.