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E-mail
3004987436@qq.com
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Phone
18117197628
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Address
B102, Yinxiao Building, Lane 36, Biquan Road, Minhang District, Shanghai
Shanghai Bohu Biotechnology Co., Ltd
3004987436@qq.com
18117197628
B102, Yinxiao Building, Lane 36, Biquan Road, Minhang District, Shanghai
The company's products are for scientific research purposes only and cannot be used for clinical diagnosis!
Product attributes
Item Number |
Product Name |
Specifications |
P-PR1017 |
Brand of endotoxin free high-purity plasmid small amount rapid extraction kit |
50 time |
Storage conditions:After receiving this product, store each component at the indicated temperature for 18 months without affecting its effectiveness.
Product Introduction
This reagent kit uses an improved SDS alkaline lysis method to lyse cells. The crude extract is filtered through a column to remove endotoxins, and then the silica matrix membrane in the centrifugal adsorption column selectively binds to plasmid DNA in the solution under high salt and low pH conditions. Impurities and other bacterial components are removed by passing protein solution and rinsing solution, and finally pure plasmid DNA is eluted from the silica matrix membrane with low salt and high pH elution buffer.
Product Features:
1. The silicon matrix membrane inside the centrifugal adsorption column is entirely made of imported specially designed adsorption membranes, with minimal differences in adsorption capacity between columns and good repeatability. Overcoming the drawbacks of unstable membrane quality in domestic reagent kits.
2. Some protein removal formulas can efficiently remove nucleases. Even strains with abundant nuclease content, such as JM series and HB101, can be easily removed. Effectively preventing plasmid degradation by nucleases.
3. The method for clearing endotoxins generally has a clearance effect of less than 0.1 EU/μ g.


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