-
E-mail
yilaibo@shyilaibo.com
-
Phone
15221734409
-
Address
B650, Block B, No. 180 Changjiang South Road, Baoshan District, Shanghai
Product Categories
Ilebo Biotechnology (Shanghai) Co., Ltd
CRISPR/Cas12a system experimental services
NegotiableUpdate on 03/04
- Model
- Nature of the Manufacturer
- Producers
- Product Category
- Place of Origin
Overview
CRISPR/Cas12a system experimental service: CRISPR/Cas12a (formerly known as Cpf1) belongs to Class 2 Type V CRISPR system, which is an adaptive immune mechanism for bacteria and archaea, used to identify and cleave invading virus or plasmid DNA. Compared with Cas9, its unique working mode gives it significant advantages in gene editing, molecular detection, and multi gene regulation.
Product Details
1CRISPR/Cas12a system experimental servicesSystem Definition and Biological Origins
CRISPR/Cas12a(Originally known asCpf1）Belonging toClass 2 Type V CRISPR SystemIt is an adaptive immune mechanism between bacteria and archaea, used to recognize and cleave invading viruses or plasmid DNA. Compared with Cas9, its unique working mode gives it significant advantages in gene editing, molecular detection, and multi gene regulation.
IICRISPR/Cas12a system experimental servicesCore molecular mechanisms and technical characteristics
（1） Function process
（2） Comparison of Key Characteristics (Cas12a vs Cas9)
| parameter | CRISPR/Cas12a | CRISPR/Cas9 |
|---|---|---|
| Protein size | 1200-1300 amino acids (smaller) | 1000-1600 amino acids |
| RNA dependence | only needcrRNA(No tracrRNA) | needcrRNA + tracrRNAOr chimeric sgRNA |
| CrRNA processing | Autonomous RNase activity (built-in processing capability) | Dependent on host RNase III and tracrRNA |
| Cutting end type | 黏性末端（5' 突出） | Flat end |
| PAM recognition sequence | 5'-TTTN/TTTV-3'(Rich in T) | 5'-NGG-3'(Rich in G) |
| Nuclease domain | singleRuvC structural domain | HNH+RuvC dual domain |
| Trans cutting activity | Yes (can non-specific cleave ssDNA) | none |
note：
VRepresenting A/C/G (non-T), such as 5 '- TTTA/TTTC/TTTG-3'.
黏性末端More likely to trigger homologous recombination repair (HDR), improving precision editing efficiency.
3、 Advantages and application scenarios
（1） Technical advantages
Efficiency of multi gene editing：
A single crRNA array can simultaneously target multiple genes without the need for repeated construction of tracrRNA, making it suitable for complex pathway regulation.
AT enriched genome adaptability：
The editing efficiency of AT rich genomes (such as plants and parasites) is significantly higher than that of Cas9.
Low off target risk：
Strictly dependent on PAM activation, and with trans cleavage activity that can be turned off, the whole genome off target rate is lower than Cas9.
Delivery convenience：
The protein is smaller and easier to package into viral vectors such as AAV, making it suitable for in vivo gene therapy.
（2） Core application areas
| Application Direction | case | Advantages reflected |
|---|---|---|
| Multi gene knockout | Simultaneous editing of 3 drought resistant genes in maize, with editing efficiency>60% | Simplified design of crRNA array |
| Accurate gene insertion | Utilizing sticky tips to enhance HDR and achieve targeted repair of human FIX coagulation genes | High fidelity repair |
| Molecular Diagnostics | Developing nucleic acid detection (CRISPR-DETECT) by combining trans cleavage activity | High sensitivity, no need for PCR amplification |
| Antiviral Research | Editing BmNPV virus receptor gene in silkworms increases antiviral efficiency by 40% compared to Cas9 | Efficient cutting of AT enrichment zone |
| Optimization of gene therapy vectors | Cas12b (smaller variant) has a 50% reduction in off target rate compared to Cas9, making it suitable for clinical use | High security delivery |
