Exonuclease T

The company's products are for scientific research purposes only and cannot be used for clinical diagnosis!

Product attributes

Product Introduction

Exo T, also known as RNase T, is a single stranded RNA or DNA specific nuclease that requires a free 3 'end to remove nucleotides in the 3' -5 'direction. Exonuclease T can be used to generate flat ends from RNA or DNA containing 3 'protruding ends.
This product is expressed through recombinationExonuclease THigh purity protein obtained from genes, free from contamination by endonucleases and other exonucleases.

Definition of Activity:The amount of enzyme required to catalyze the production of 0.1 nmol of TCA soluble DNA from 1 nmol [3H] - labeled polythymidine in a 100 μ l reaction system at 25 ℃ for 30 minutes is defined as one active unit.
1XExonuclease TBuffer
50mM KAc，20mM Tris-Ac，10mM Mg(Ac)2，1mM DTT（pH 7.9）
Thermal deactivation:65°C，20min。
Enzyme storage solution:10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1mM EDTA, 50% Glycerol, 200 μg/ml BSA, pH 7.5。
storageStored at -20 ° C for 2 years to avoid repeated freezing and thawing.
Precautions
Exonuclease T has different activities towards RNA and DNA. For RNA, under standard reaction conditions, 1 unit of exonuclease T can digest 1.0 pmol of rA20 through gel electrophoresis detection.

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