Fc γ R Ⅱ/CD32 ELISA enzyme-linked immunosorbent assay kit

Fc γ R Ⅱ/CD32 ELISA enzyme-linked immunosorbent assay kitPlasma:
EDTA, sodium citrate, or heparin should be selected as anticoagulants according to the requirements of the reagent kit. 0% (v/v) anticoagulant (0. M sodium citrate or% heparin or 2.0% EDTA. Na2) should be added and mixed for 0-20 minutes, followed by centrifugation for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant. If precipitation forms, it should be centrifuged again.
Urine, ascites, cerebrospinal fluid: Collect with sterile tubes, centrifuge for about 20 minutes (2000-3000 rpm), and carefully collect the supernatant. If precipitation forms, it should be centrifuged again.
G Fc region receptor II (Fc γ R II/CD32) ELISA enzyme-linked immunosorbent assay kit product abbreviation: English name: Human Receptor II for the Fc region of immunoglobulin G. Fc γ R II ELISA Kit is available in stock, and quality issues can be exchanged or returned, eliminating your worries. All ELISA kits are free of charge for testing, with full Elisa experimental technical guidance and free experimentation. G Fc segment receptor II (Fc γ R II/CD32) ELISA detection kit 48T/96T.
Self provided items:
37 ℃ constant temperature box
2. Standard specification enzyme-linked immunosorbent assay (ELISA) reader
3. Precision pipette and disposable suction head
4. Distilled water
5. Disposable test tube
6. Absorbent paper
Fc γ R Ⅱ/CD32 ELISA enzyme-linked immunosorbent assay kitResearch ELISA kit:
Species: humans, rats, mice, guinea pigs, hamsters, nude mice, rabbits, pigs, dogs, monkeys, horses, cows, sheep, chickens, ducks, fish, etc.
Specimen: serum, plasma, cell supernatant, urine, body fluids, lavage fluid, cerebrospinal fluid, atrial fluid, pleural fluid, tissue, etc.
）Serum:
After natural coagulation of blood at room temperature for 0-20 minutes, centrifuge for about 20 minutes (2000-3000 revolutions per minute). Carefully collect the supernatant. If precipitation forms during storage, it should be centrifuged again.
2) Plasma:
EDTA, sodium citrate, or heparin should be selected as anticoagulants according to the requirements of the specimen. After mixing for 0-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant. If precipitation forms during storage, it should be centrifuged again.
3) Urine:
Collect with sterile tubes. Centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant. If precipitation forms during storage, it should be centrifuged again. Follow this procedure for pleural effusion, ascites, and cerebrospinal fluid.
4) Cell culture supernatant:
A、 When detecting secretory components, collect them using sterile tubes. Centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant.
B、 When detecting the components inside cells, dilute the cell suspension with PBS (pH 7.2-7.4) to a cell concentration of around 1 million/ml. By repeatedly freezing and thawing, cells are destroyed and intracellular components are released. Centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant. If precipitation forms during storage, it should be centrifuged again.
5) Organizational specimen:
After cutting the specimen, weigh it. Add a certain amount of PBS, pH 7.4. The enzyme-linked immunosorbent assay kit is rapidly frozen and stored in liquid nitrogen for future use. The specimen remains at a temperature of 2-8 ℃ even after melting. Add a certain amount of PBS (pH 7.4) and homogenize the specimen thoroughly by hand or using a homogenizer. Centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant. After packaging, one portion is to be tested, and the rest is to be frozen for future use.
Sample collection:
Before collecting specimens, it is necessary to clarify whether the components to be tested are stable enough. Samples collected for testing on the same day should be stored at 4 ℃ for future use. If there are special reasons that require periodic collection of samples, they should be promptly packaged and stored at -20 ℃ or -70 ℃. Avoid repeated freezing and thawing. The specimen can be stored for 48 hours at 2-8 ℃ and for months at -20 ℃. -70 degrees can be stored for 6 months. Some * * * specimens need to be added.
The impact of genetic engineering reagents on the development of immunoassay technology:
The foundation of immunoassay technology lies in the specific binding reaction between antigens and antibodies, so any high-quality diagnostic reagent cannot do without high-quality raw materials such as antigens, antibodies, enzymes, etc. Previously, antigens used for immunoassays were usually various purified antigens, while antibodies were polyclonal antibodies obtained by immunizing animals with purified antigens and monoclonal antibodies obtained by hybridoma technology. Antigen or antibody markers such as enzyme-linked immunosorbent assay (ELISA) conjugates were prepared by various total chemical synthesis methods. In recent years, with the development of molecular biology, the use of genetic engineering methods to prepare various special antigens or antibodies and their enzyme conjugates for immunoassay has become a new generation of reagents, and various new types of assay methods are constantly emerging.

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hirudin113274-56-9≥95（PAGE）,1200ATU/g

mussel adhesive protein3047117-55-2≥95（PAGE）

Superoxide dismutase (SOD)9054-89-1≥ 95 (PAGE), enzyme activity: 10000 U/g

phytosterol949109-75-5Purity>98

squalane111-01-3Purity>98

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Recombinant human epidermal growth factor62253-63-8Purity greater than 98

ecdysone5289-74-7Purity greater than 98

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