Instruction manual for residual ELISA reagent kit

Instruction manual for residual ELISA reagent kit
GOY-E630
It can be used for qualitative and quantitative detection of residual amounts in animal tissues (muscle, liver, fish, shrimp, etc.), honey, milk, formula, ice cream, cream, and vaccine samples.
【 Experimental Principle 】 This reagent kit adopts the indirect competitive ELISA method. Conjugated antigens are pre coated on the microplate strips of the enzyme-linked immunosorbent assay (ELISA) plate. The residual antibodies in the sample compete with the pre coated conjugated antigens on the microplate strips of the ELISA plate. After adding the enzyme-linked immunosorbent assay (ELISA) secondary antibody, TMB substrate is used for color development. The absorbance value of the sample is negatively correlated with the content of residual substances. The residual amount in the sample can be obtained by comparing it with the standard curve and multiplying it by the corresponding dilution factor.
Please refer to the instruction manual for details

The box contains reagents:
The reagent kit has been used 96 times. Store at 2-8 ° C.
2 * 8 microporous plates coated with conjugate.
2. Standard sample (0; 4; 0; 40; 00; 400 ng/mL): 6 vials, each bottle contains. 0ml, red, ready to use.
3. Anti antibody (mouse): 6ml, red, ready to use type
4 Conjugate (anti-mouse-IgG-HRP)：5 mL, Red, ready to use type.
5. Substrate solution (TMB): 5ml, pre dyed red, ready to use.
6. Termination solution (0.5 M H2SO4): 5 mL, ready to use.
7. Sample diluent (Tris): 2 x 50 mL, 0x concentrated solution, red. Dilute with 9 distilled water. The diluent should be stored at 4 ° C for at least a week. If precipitation occurs during the refrigeration crystallization process, the concentrate should be heated to 37 ℃ for 5 minutes.
8. Washing solution (PBS+Tween 20): 60 mL of 0x concentrated solution. Dilute with 9 distilled water. The diluent should be stored at 4 ° C for at least a week. If precipitation occurs during the refrigeration crystallization process, the concentrate should be heated to 37 ℃ for 5 minutes.
9. Two plastic films are covered on the microplate.
0. Plastic bags are stored without using microplates.
Residual ELISA reagent kit manual and operation manual.

hirudin113274-56-9≥95（PAGE）,1200ATU/g

mussel adhesive protein3047117-55-2≥95（PAGE）

Superoxide dismutase (SOD)9054-89-1≥ 95 (PAGE), enzyme activity: 10000 U/g

phytosterol949109-75-5Purity>98

squalane111-01-3Purity>98

Nattokinase133876-92-3≥95（PAGE）,20000FU/g

Recombinant human epidermal growth factor62253-63-8Purity greater than 98

ecdysone5289-74-7Purity greater than 98

Water-soluble silk fibroin protein/Purity greater than 98, molecular weight 1000Da

Ergothioneine497-30-3Purity greater than 98

250g of Cinnamomum sulfate tryptone broth (LST) was used for the determination of coliforms and fecal coliforms by multi tube fermentation method (GB 4789.3-200, GB/T 4789.38-2008, GB/T 4789.39-200)
250g lactose peptone culture medium is used for the determination of coliforms in water by multi tube fermentation or membrane filtration method (GB/T5750.2-2006 and GB/T8538-2008).
250g of bright green lactose culture medium (BGB) is used for the confirmation test of coliform bacteria in drinking mineral water by multi tube fermentation method (GB/T8538-2008).
250g bile lactose medium (BL) is used for the enrichment culture of Escherichia coli and Pseudomonas aeruginosa in pharmaceuticals.
250g of intestinal bacterial enrichment broth is used for the cultivation of intestinal bacteria
250g of fuchsin sodium sulfite medium (Endo agar medium) is used for the isolation or confirmation test of Escherichia coli in water.
250g of MacConkey agar medium (pharmacopoeia) is used to isolate Gram negative intestinal bacteria for lactose fermentation.
250g of Eosin Agar Medium (EMB) is used for the detection of Escherichia coli and Salmonella in pharmaceuticals.
250g MacConkey agar medium for isolating and fermenting lactose from Gram negative intestinal bacteria
Improved Eosin Blue Agar 250g for isolation and cultivation of Gram negative intestinal bacteria.
Yihongmei Blue Agar (EMB) 250g is used for isolating Gram negative intestinal bacteria, especially coliforms and fecal coliforms
Aliz gal agar 00g for rapid detection of coliform bacteria on agar plates
MUGal (4-methylumbelliferone - β - galactoside) broth 00g for rapid detection of coliforms using multi tube fermentation method
Confirmation Test for Determination of Coliform in Food, Purified Water, and Disposable Sanitary Products by Multi tube Fermentation Method Using 250g Lactose Fermentation Medium
Confirmation Test for Determination of Coliform in Food, Purified Water, and Disposable Sanitary Products by Multi tube Fermentation Method Using 250g Lactose Fermentation Medium
250g lactose bile salt fermentation medium is used for the determination of coliform bacteria, fecal coliform bacteria, and fecal coliform bacteria in food, medicine, purified water, mineral water, and disposable sanitary products by multi tube fermentation method ..
Add 0 pieces/box of modified MSRV culture medium matching reagents to the modified MSRV culture medium agar base (023025) to make modified MSRV culture medium agar
Improved MSRV agar base 250g for isolating mobile Salmonella (Merck method)
Add 0 bottles of Shigella enriched broth reagent to 225mL of Shigella enriched broth (0234) each.

The operation process is as follows:
Add 00 μ l of the test sample to each well of the test sample, and set 3 parallel wells for each type of sample; Set up two negative control wells and add 00 μ l of untreated cell lysate to each well; Set up another blank control well and add 00 μ l of pure cell lysate.
(2) Place the enzyme-linked immunosorbent assay (ELISA) plate at 4 ℃ and coat overnight.
(3) Plate washing: Absorb the reaction solution in the well, rinse it once with washing solution (after filling the plate well with washing solution, shake it off), then fill the plate well with washing solution, soak for -2 minutes, and shake intermittently. Shake off the liquid inside the hole and pat dry on absorbent paper. Repeat washing 3-4 times.
(4) Add 50 μ l of PBS to each negative control well, and add 50 μ l of 500 diluted rabbit anti human AIF antibody working solution to each sample well and blank well.
(5) Place the enzyme-linked immunosorbent assay (ELISA) plate in a wet box at 37 ℃ and incubate for 60 minutes.
(6) Wash the board, same as (4).
(7) Add 00 μ l of HRP labeled goat anti rabbit antibody working solution diluted with 5000 to each well.
(8) Place the enzyme-linked immunosorbent assay (ELISA) plate in a wet box at 37 ℃ and incubate for 60 minutes.
(9) Wash the board, same as (4).
(0) Add 00 μ l of TMB chromogenic solution to each well, gently mix for 0s, and react in the dark at 37 ℃ for 5-20min.
Add 00 μ l of 2mol/L H2SO4 to each well to terminate the reaction.
(2) Measure the absorbance values W and W2 at 450nm and 630nm respectively, and the final measured OD value is the difference between the two (W-W2) to reduce light interference caused by scratches or fingerprints on the container.
(3) Data processing: After obtaining the OD values of the specimen (S) and negative control (N), calculate the S/N value. S/N ≥ 2 is the positive judgment criterion.