PCDH1 human procalcitonin Sig enzyme-linked immunosorbent assay kit

The experimental principle of the company's ELISA reagent kit is available in stock, and any quality issues can be exchanged or refunded, eliminating your worries. All ELISA reagent kits are available for free testing, full Elisa experimental technical guidance, and free laboratory experiments.

Usage: Special reagents for scientific research and experimentation, not for clinical diagnosis.
Specimen: serum, plasma, cell supernatant, urine, body fluids, lavage fluid, cerebrospinal fluid, atrial fluid, pleural fluid, tissue, etc.
Species: humans, rats, mice, rabbits, pigs, dogs, monkeys, horses, cows, sheep, chickens, ducks, fish, etc.
In terms of transportation: spot supply, usually by express delivery, arriving the next day in Jiangsu, Zhejiang, and Shanghai, and taking three to five days to arrive in other regions and remote areas
常见ELISA试剂盒系列: IL-1、IL-1α、IL-1β、IL-2、IL-2R、IL-27、IL-23、IL-17、IL-18、IL-9、IL-8、IL-6、IL-5、IL-4、IL-2、IL-13、IL-11、IL-10、IL-12P70、IL-12P40、IFN-ω、IFN-β、IFN-α、 值 IFN-γ、TNF-β、TNF-α、sICAM-1、LEP、MMP-3 等.

Experimental procedure:
1. Preparation of standards, reagents, and samples before the experiment;
2. Add 100 µ L of standard and sample, incubate at 37 ° C for 1 hour;
3. Discard and add detection solution A100 µ L, incubate at 37 ° C for 1 hour;
4. Wash the board three times;
5. Add detection solution B100 µ L and incubate at 37 ° C for 30 minutes;
6. Wash the board 5 times;
7. Add 90 µ L of TMB substrate and incubate at 37 ° C for 10-20 minutes;
8. Add 50 µ L of termination solution and immediately take a 450nm reading.
Sample processing and requirements:
1. Serum: Blood naturally coagulates at room temperature for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 revolutions per minute).
Carefully collect the supernatant. If precipitation occurs during storage, centrifuge again.
2. Plasma: EDTA or sodium citrate should be selected as anticoagulants according to the requirements of the specimen. After mixing for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant. If sediment forms during storage, it should be centrifuged again.
3. Urine: Collect with a sterile tube and centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant. If sediment forms during storage, centrifuge again. Refer to the implementation for pleural effusion, ascites, and cerebrospinal fluid.
4. Cell culture supernatant: When detecting secreted components, collect them using sterile tubes. Centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant. When detecting intracellular components, dilute the cell suspension with PBS (pH 7.2-7.4) to a cell concentration of around 1 million/ml. By repeatedly freezing and thawing, cells are destroyed and intracellular components are released. Centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant. If precipitation forms during storage, it should be centrifuged again.
5. Tissue specimen: After cutting the specimen, weigh it. Add a certain amount of PBS, pH 7.4. Quickly freeze and store in liquid nitrogen for future use. The specimen remains at a temperature of 2-8 ℃ even after melting. Add a certain amount of PBS (pH 7.4) and homogenize the specimen thoroughly by hand or using a homogenizer. Centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant. After packaging, one portion is to be tested, and the rest is to be frozen for future use.
6. Extract the specimen as soon as possible after collection, according to relevant literature, and conduct experiments as soon as possible after extraction. If the experiment cannot be conducted immediately, the specimen can be stored at -20 ℃, but repeated freezing and thawing should be avoided
7. Samples containing NaN3 cannot be detected because NaN3 inhibits the activity of horseradish peroxidase (HRP).
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Notes:
1. Please store at 2-8 ℃ before use. If the standard product after reconstitution is not used up, please dispose of it.
2. Turbulence and possible inversion during the transportation of trace reagents can cause the liquid to stick to the tube wall or bottle cap. Therefore, please rotate 1000 revolutions per minute before use to allow the liquid adhering to the tube wall or bottle cap to settle to the bottom of the tube.
3. The concentrated washing solution taken out of the refrigerator may have crystals, which is a normal phenomenon. Heat it slightly to 40 ℃ to dissolve the crystals before preparing the washing solution.
(Heating temperature should not exceed 50 ℃) When using, the detergent should be at room temperature.
4. Avoid mixing components of different batch numbers of reagent kits (except for washing solution and reaction termination solution).
5. Adequate and slight mixing is particularly important for the reaction results. Use a micro oscillator (using the lowest frequency). If there is no micro oscillator, gently mix by hand before incubation.
6. It is recommended to perform double well testing for standard and sample testing during the experiment.
7. The test tubes and suction tips used in the experiment are disposable and must not be mixed between different liquids! Otherwise, it will affect the test results.
8. Please wear test clothes and latex gloves for protection during the experiment. Especially when testing blood or other body fluid specimens, please follow the safety protection regulations of the national biological laboratory.