Plant nitrate nitrogen test kit visible spectrophotometry method

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Product Name:Plant nitrate nitrogen test kit visible spectrophotometry method
Product Specifications100 tubes/96 samples

Detection method: Visible spectrophotometry

Product Code:LZ-01497S

Product Category: Nitrogen Metabolism Series
Product Introduction:

Sample preparation:
1）. Directly or diluted use of clear, colorless, neutral liquid samples, with a volume of up to 2.000ml.

2）. Filter the turbid solution.

3）. Remove CO2 from the sample (filtered according to the instructions of the fructose content test kit).

4 ）. Instructions for Fructose Content Test Kit: Adjust the pH of the acidic sample to 8.0 by adding potassium hydroxide or sodium hydroxide.

5 ）. Adjust the pH value of the acidic light colored sample to 8.0 and incubate for about 15 minutes.

6 ）. Use blank samples as controls to determine colored samples (adjust pH to 8.0 if necessary).

7 ）. Treat dark undiluted or larger volume samples with PVPP (polyvinylpyrrolidone) or polyamide.

8 ）. Crush and stir solid or semi-solid samples, dissolve and extract with water.

9 ）. Use Carrez reagent to deprotein samples containing proteins.

10）. Extract samples containing fat with hot water.

Features:
（1) Widely used

Due to the absorption of various inorganic and organic substances in the UV visible region, they can be measured using this method. So far, almost all elements on the periodic table of chemical elements (except for a few radioactive and inert elements) can be treated using this method.

（2) High sensitivity

Due to the development of corresponding disciplines, the synthesis and research of new organic colorants have made remarkable progress, greatly improving the sensitivity of element determination. Especially due to the application research of multi-component complexes and various surfactants, the molar absorptivity of many elements has increased from tens of thousands to hundreds of thousands. Compared to other trace analysis methods, the precision and accuracy of spectrophotometry are relatively high. Not only is photometry widely used in practical work, but it is also highly valued in the development of standard reference materials, and many photometric analysis methods have been developed as standard methods.

（3) Good selectivity

At present, some elements can be directly determined by photometry by controlling appropriate color conditions, such as cobalt, uranium, nickel, copper, silver, iron, etc. There are already satisfactory methods for the determination of these elements.

（4) High accuracy

For general spectrophotometry, the relative error in concentration measurement isWithin the range of 1-3%, if differential spectrophotometry is used for measurement, the error can often be reduced to a few thousandths.

（5) Wide applicable concentration range

Can be obtained from constants（1-50% (especially using differential methods) to trace amounts (10-6-10-8%) (after pre enrichment).

（6) Low analysis cost, easy to operate, and fast.

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Operation process:
1. Take out the required Flat noodles from the aluminum foil bag after 20 min of room temperature balance, and seal the remaining Flat noodles with a self sealing bag and put it back at 4 ℃.

2. Set up standard wells and sample wells, and add 50 μ L of standard samples of different concentrations to each standard well;

3. Add 50 μ L of the sample to be tested into the sample well; Blank holes are not added.

4. Except for blank wells, add 100 μ L of horseradish peroxidase (HRP) labeled detection antibody to each well of the standard and sample wells, seal the reaction well with a sealing plate membrane, and incubate at 37 ℃ in a water bath or constant temperature box for 60 minutes.

5. Discard the liquid, pat dry on absorbent paper, fill each well with washing solution (350 μ L), let it stand for 1 minute, shake off the washing solution, pat dry on absorbent paper, repeat washing the board 5 times (or use a washing machine to wash the board).

6. Add 50 μ L of substrate A and B to each well, and incubate at 37 ℃ in the dark for 15 minutes.

7. Add 50 μ L of termination solution to each well and measure the OD value of each well at a wavelength of 450nm within 15 minutes.