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Shandong Yuntang Intelligent Technology Co., Ltd

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    413230237@qq.com

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    13371098225

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    Room 415, Building 1, Optoelectronic Industry Accelerator (Phase I), Yuqing Community, Xincheng Street, Weifang High tech Zone, Shandong Province

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Qualitative 96 well PCR instrument

NegotiableUpdate on 03/06
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Overview
The qualitative 96 well PCR instrument is mainly used to run real-time fluorescence quantitative PCR experiments. Through fluorescence excitation and collection, the experimental process is monitored in real time. The experimental process data is plotted as a fluorescence curve and presented in real time on the instrument display interface. The experimental data is fitted and analyzed, and finally a PDF format experimental report can be generated.
Product Details

1、 Product Overview:
Qualitative 96 well PCR instrumentMainly used for running real-time fluorescence quantitative PCR experiments, monitoring the experimental process in real time through fluorescence excitation and acquisition, plotting the experimental process data into fluorescence curves, presenting them in real-time on the instrument display interface, fitting and analyzing the experimental data, and finally generating PDF format experimental reports.
Rapid and accurate qualitative and quantitative analysis of target nucleic acids contained in biological samples (such as blood, body fluids, etc.) taken from other animals, as well as standard curve, melting curve, genotyping and other analyses of special samples.
2、 Basic principles
Qualitative 96 well PCR instrumentPrinciple: Polymerase chain reaction (PCR) is a molecular biology technique used to amplify and amplify specific DNA fragments. It can be seen as a special DNA amplification outside the body, consisting of several steps such as high-temperature denaturation, low-temperature annealing, and appropriate temperature extension to form a temperature cycle, allowing the target gene to be rapidly amplified. It has the characteristics of strong specificity, high sensitivity, easy operation, and time-saving, and is a major innovation in gene amplification technology. PCR technology can amplify extremely small amounts of target DNA by millions of times, greatly improving the analysis and detection capabilities of DNA molecules.
Real time fluorescence quantitative PCR technology is a method that adds corresponding fluorescent dyes or fluorescent labeled probes on the basis of conventional PCR, and detects the entire PCR process in real time through changes in fluorescence signals during the PCR reaction process. The method uses fluorescent chemicals to monitor the total amount of products after each polymerase chain reaction (PCR) cycle, and quantitatively analyzes specific DNA sequences in the test sample through internal or external reference methods.
4、 Application Fields
□ Basic scientific research
□ Pathogen detection
□ Animal epidemic detection
□ Meat products adulterated
□ Transgenic testing
□ Food hygiene quarantine
□ Drug development and rational drug use
□ Gene expression
□ Water monitoring

5、 Product Features
The thermoelectric refrigeration system used has higher heating and cooling efficiency
The constant current power supply and 6-zone independent temperature control method fully ensure the stable performance of the system. The light source and optical path design make the fluorescence more realistic and reliable
Independent fluorescent channels reduce crosstalk between different channels
Comprehensive analysis functions combined with custom format report functions meet the needs of customers in different scenarios. The software is simple and intuitive to operate, easy to learn and use


Product Model

YT-PC96

Sample capacity

96 holes * 0.2ml

Sample volume

20-120μL

Applicable consumables

0.2ml single tube, 8x0.2ml discharge tube, 96 well plate

Temperature control range

0-99°C

Gradient temperature control

6-module gradient temperature control, with a maximum temperature difference support of 25..

heating rate

最大 7 °C/s, 平均 3.8°C/s

降温速率

最大4.5 °C/s , 平均3.5°C/s

Temperature control accuracy

±0.1 °C

Temperature uniformity

±0.1 °C

detection sensitivity

Can detect single copy genes

dynamic range

10-10A10

repetitiveness

CV<3%

Related Linear

0.99-1

amplification efficiency

99%-101%

analysis algorithm

Amplification curve, standard curve quantification, melting curve, genotyping SNP (optional)

TouchDown function

Support time and temperature increasing and decreasing modes

test kit

Custom temperature running program, open fluorescent quantitative PCR reagent

light source

4 long-life LED lights with different filters that can specifically and efficiently excite fluorescent molecules in different wavelength bands

Communication medium

High temperature resistant professional optical fiber

Applicable dyes/probes

FAM, SYBR- Green; HEX,JOE,VIC,TET; ROX,Texas-Red; CY5

Dimensions & Weight

410*320*196 mm, 11 kg

power supply

220V

operating system

Standard external Wi Fi Windows computer (Wi Fi 7 system or above), optional Wi Fi Windows tablet integrated