Rat femoral artery endothelial cells

① Organizational block cultivation method

Tissue block culture is a commonly used, simple, and highly successful primary culture method. The basic method is to inoculate the cut small tissue clusters into a culture bottle (or dish), and the bottle wall can be pre coated with a thin layer of collagen to facilitate the adhesion of the tissue blocks to the bottle wall, allowing surrounding cells to grow outward along the bottle wall.

② Digestive cultivation method

③ Suspension cell culture method

For cells that grow in suspension, such as leukemia cells, lymphocytes, bone marrow cells, cancer cells and immune cells in pleural and ascites, digestion is not required. They can be isolated by low-speed centrifugation and cultured directly, or cultured by inoculating after lymphocyte stratification.

④ Organ culture

Organ culture refers to the direct cultivation of organs or tissue blocks obtained from donors under specific environmental conditions outside the body without tissue separation. Organ culture can maintain the relative integrity of organ tissues and can be used to focus on observing the connections, arrangements, and interactions between cells, as well as the biological regulatory effects of local environments.

Product Name:Rat femoral artery endothelial cells

English nameRat femoral artery endothelial cells

Organizational source:Femoral artery tissue

Product Specifications5×105cells/T25Cell culture bottle

Cell Introduction:

The femoral artery is the main trunk of the lower limb arteries, which continues from the external iliac artery. Insert a triangle deep into the midpoint of the inguinal ligament. In the femoral triangle, the femoral artery is first located on the outer side of the femoral vein, gradually crossing from the outer side to the front of the femoral vein, descending into the adductor tube, and then passing through the adductor tendon hiatus to the popliteal fossa, renamed as the popliteal artery.

The femoral artery is located superficially at the midpoint of the groin and can be palpated for pulsation. It is the site for emergency compression hemostasis and puncture in clinical practice. The endothelial cells of the femoral artery form the inner wall of the artery and are continuously affected by blood flow shear stress. Under the action of shear stress, endothelial cells secrete different endothelial factors, which in turn affect vascular contraction and growth.

Aortic endothelial cells also regulate the expression of cell adhesion molecules to control and precisely regulate inflammatory responses and tissue fibrosis. The primary femoral artery endothelial cells cultured in vitro can effectively help researchers to study the mechanism of endothelial dysfunction, the pathogenesis of arterial atherosclerosis, and develop new methods.

Cell characteristics：

1）The organization is derived from normal femoral artery tissue of experimental animals.

2)Cell identification: Von Willebrand factor（vWF）Fluorescence staining is positive.

3)After identification, the purity of the cells was found to be higher than90%.

4)does not containHIV-1TheHBVTheHCVMycoplasma, yeast, and.

5)Cell growth mode: cobblestone shaped cells, irregular cells, adherent culture.

Recommended culture medium:

We recommend usingDelfPrimary endothelium cell cultivation system As a culture medium for in vitro cultivation.

Sampling → Separation → Cultivation and Maintenance

1. Material selection

The collection of human and animal cells is the primary condition for successful primary cell culture, which directly affects the in vitro culture of cells.

(1) Basic requirements for material selection

① Attention should be paid to freshness and freshness when selecting materials

② Strictly sterile

③ Prevent mechanical damage

④ Remove useless tissues and avoid drying

⑤ Attention should be paid to organizational type, degree of differentiation, age, etc

⑥ Keep good records

2. Separation

Due to the tight binding of multiple cells in the human or animal body (or embryonic tissue), it is not conducive to the growth and reproduction of each cell in vitro culture. Existing tissue blocks must be fully dispersed to dissociate the cells.

(1) Separation method of suspended cells

If the tissue material comes from suspension materials of blood, amniotic fluid, pleural fluid or ascites, the method is to use low-speed centrifugation at 1000r/min for 10 minutes. After centrifugation, different layers can be formed in the stratified solution due to the varying densities of various cells, allowing for the harvesting of target cells as needed.

(2) Separation methods for physical organizational materials

For solid tissue materials, due to the tight binding between cells, in order to fully disperse the cells in the tissue and form a cell suspension, mechanical dispersion (physical lysis) and digestion separation methods can be used.

① Mechanical dispersion method

Characteristics: Simple and fast, but it causes significant mechanical damage to tissues and has poor cell dispersion effect. It is suitable for treating soft tissues with low fiber components.

② Digestion separation method

The tissue digestion method involves cutting the tissue into smaller clumps (or paste), using enzymatic biochemical and non enzymatic chemical reactions to further loosen the bridging structure between cells, causing the clumps to swell and transform from clumps to flocs. At this point, mechanical methods are used, such as blowing and dispersing with a straw, stirring with an electric magnetic stirrer, or shaking in a shaking ball bottle, to fully disperse the cell clumps, resulting in a small number of cell clusters and a large number of individual cell suspensions. After inoculation and culture, the cells are easily attached to the wall for growth.

Rat cell chorionic villi protein(CVL)Test kit, English name:CVL ELISA Kit

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Mousecardiacanscriptionfactor-GATA4ELISAKitMouse cardiac transcription factorGATA4Test kit96T/48TImport packaging

Cliakihsp- 60 elistsRat heat shock protein60

cellLYNBKinase activity quantitative detection kit(A/B/C)20time

ELISAKitIL-1β - rat interleukin1B

COL4A3Recombinant ratsCOL4A3protein(Fclabel) Protein

ENPP3/CD203c(ectonucleotide pyrophosphatase/phosphodiesterase 3 0.5mgENPP3/CD203c(ectonucleotide pyrophosphatase/phosphodiesterase 3) ENPP3antigen

CLEC3BReorganized personCLEC3B / TetranectinproteinProtein

ENTPD5 Protein HumanReorganized personEntpd 5protein

CES5A Protein MouseRecombinant miceCES5 / Carboxylesterase-5protein

ENPP3/CD203c(ectonucleotide pyrophosphatase/phosphodiesterase 3 0.5mgENPP3/CD203c(ectonucleotide pyrophosphatase/phosphodiesterase 3) ENPP3antigen

ENTPD5 Protein HumanReorganized personEntpd 5protein

COL4A3Recombinant ratsCOL4A3protein(Fclabel) Protein

CES5A Protein MouseRecombinant miceCES5 / Carboxylesterase-5protein

CLEC3BReorganized personCLEC3B / TetranectinproteinProtein

Mouse methyltransferase(Methylase)ELISAtest kit96T/48T

Human killer cell lectin like receptor (KLR) ELISA KitHuman killer cell lectin like receptor(KLR)Test kit

Humaecombinationactivatinggene2,RAG-2ELISAKitRecombinant activated genes in humans2(RAG-2)Test kit96T/48TImport packaging

Humanai-gliadinaibody,AGADetection kit for human anti gliadin/AGA IgG(AGA)Specification of detection kit:96T/48T

Plants rely on(lysine)Content Fluorescence Quantitative Detection Kit20time

HumanVitaminB12，VB12ELISAKitpersonB12(VB12)Specification of detection kit:96T/48T

Rat femoral artery endothelial cellsMouse complement1Inhibitor antibody(C1INH)Test kit 96T/48T test kit assembly/genuine

Mouse dehydrogenaseE1(PDHE1)Test kit 96T/48T test kit assembly/genuine

Mouse kinaseM2Isoenzyme type(M2-PK)Test kit 96T/48T test kit assembly/genuine

Mouse kinase(PK)Test kit 96T/48T test kit assembly/genuine

Depending on the weather conditions and transportation distance, the company and the customer will negotiate and choose one of the following methods.

1) 1mL cryopreserved cell suspension is put into 1.8ml cryopreserved tube and transported in foam insulation box filled with dry ice; After receiving the cells, please thaw and revive them for cultivation as soon as possible. If resuscitation cannot be performed immediately, the frozen cells can be stored at -80 ℃ for one month.

The T-25 culture bottle is filled with culture medium and transported at room temperature; After receiving the cells, please observe the growth status of the cells under a microscope. If the bottle laying rate exceeds 85%, immediately perform passaging operation. If there are many suspended cells, please place the culture bottle in the incubator overnight to help non dead suspended cells adhere to the wall again.

All products of our company are only used for non-medical purposes such as scientific research or industrial research, and cannot be used for clinical diagnosis or treatment of humans or animals. They are not medicinal or edible