Rat hepatic oval cells

① Organizational block cultivation method

Tissue block culture is a commonly used, simple, and highly successful primary culture method. The basic method is to inoculate the cut small tissue clusters into a culture bottle (or dish), and the bottle wall can be pre coated with a thin layer of collagen to facilitate the adhesion of the tissue blocks to the bottle wall, allowing surrounding cells to grow outward along the bottle wall.

② Digestive cultivation method

③ Suspension cell culture method

For cells that grow in suspension, such as leukemia cells, lymphocytes, bone marrow cells, cancer cells and immune cells in pleural and ascites, digestion is not required. They can be isolated by low-speed centrifugation and cultured directly, or cultured by inoculating after lymphocyte stratification.

④ Organ culture

Organ culture refers to the direct cultivation of organs or tissue blocks obtained from donors under specific environmental conditions outside the body without tissue separation. Organ culture can maintain the relative integrity of organ tissues and can be used to focus on observing the connections, arrangements, and interactions between cells, as well as the biological regulatory effects of local environments.

Product Name:Rat hepatic oval cells

English namePrimary rat hepatic oval cells

Organizational source:liver tissue

Product Specifications5×105cells/T25Cell culture bottle

Cell Introduction:

Hepatic oogonia are a type of stem cell in the liver with strong proliferation and differentiation potential. After liver damage and loss, the liver has strong regeneration and repair abilities due to the role of stem cells in the liver. When the liver suffers severe acute injury, hepatic oval cells are activated and can differentiate into various functional cells such as hepatic parenchymal cells and intrahepatic bile duct epithelium, repairing liver damage.

The hepatic oval has two sources: intrahepatic and extrahepatic. In addition, there is a close relationship between hepatic oval cells and the occurrence of primary liver cancer, and the study of hepatic oval cells is of great significance in multiple aspects.

Cell characteristics：

1） The tissue is derived from the liver tissue of treated rats.

2)Cell identification:c-kitorAFPFluorescence staining is positive.

3)After identification, the purity of the cells was found to be higher than90%.

4)does not containHIV-1TheHBVTheHCVMycoplasma, yeast, and.

5)Cell growth mode: polygonal cells, adherent culture.

Recommended culture medium:

We recommend usingDelfPrimary hepatic oval cell cultivation system As a culture medium for in vitro cultivation.

Sampling → Separation → Cultivation and Maintenance

1. Material selection

The collection of human and animal cells is the primary condition for successful primary cell culture, which directly affects the in vitro culture of cells.

(1) Basic requirements for material selection

① Attention should be paid to freshness and freshness when selecting materials

② Strictly sterile

③ Prevent mechanical damage

④ Remove useless tissues and avoid drying

⑤ Attention should be paid to organizational type, degree of differentiation, age, etc

⑥ Keep good records

2. Separation

Due to the tight binding of multiple cells in the human or animal body (or embryonic tissue), it is not conducive to the growth and reproduction of each cell in vitro culture. Existing tissue blocks must be fully dispersed to dissociate the cells.

(1) Separation method of suspended cells

If the tissue material comes from suspension materials of blood, amniotic fluid, pleural fluid or ascites, the method is to use low-speed centrifugation at 1000r/min for 10 minutes. After centrifugation, different layers can be formed in the stratified solution due to the varying densities of various cells, allowing for the harvesting of target cells as needed.

(2) Separation methods for physical organizational materials

For solid tissue materials, due to the tight binding between cells, in order to fully disperse the cells in the tissue and form a cell suspension, mechanical dispersion (physical lysis) and digestion separation methods can be used.

① Mechanical dispersion method

Characteristics: Simple and fast, but it causes significant mechanical damage to tissues and has poor cell dispersion effect. It is suitable for treating soft tissues with low fiber components.

② Digestion separation method

The tissue digestion method involves cutting the tissue into smaller clumps (or paste), using enzymatic biochemical and non enzymatic chemical reactions to further loosen the bridging structure between cells, causing the clumps to swell and transform from clumps to flocs. At this point, mechanical methods are used, such as blowing and dispersing with a straw, stirring with an electric magnetic stirrer, or shaking in a shaking ball bottle, to fully disperse the cell clumps, resulting in a small number of cell clusters and a large number of individual cell suspensions. After inoculation and culture, the cells are easily attached to the wall for growth.

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Depending on the weather conditions and transportation distance, the company and the customer will negotiate and choose one of the following methods.

1) 1mL cryopreserved cell suspension is put into 1.8ml cryopreserved tube and transported in foam insulation box filled with dry ice; After receiving the cells, please thaw and revive them for cultivation as soon as possible. If resuscitation cannot be performed immediately, the frozen cells can be stored at -80 ℃ for one month.

The T-25 culture bottle is filled with culture medium and transported at room temperature; After receiving the cells, please observe the growth status of the cells under a microscope. If the bottle laying rate exceeds 85%, immediately perform passaging operation. If there are many suspended cells, please place the culture bottle in the incubator overnight to help non dead suspended cells adhere to the wall again.

All products of our company are only used for non-medical purposes such as scientific research or industrial research, and cannot be used for clinical diagnosis or treatment of humans or animals. They are not medicinal or edible