TRzol (Total RNA Extraction Reagent)

The company's products are for scientific research purposes only and cannot be used for clinical diagnosis!

Product attributes

Product Introduction

TRzol reagent is a reagent that directly extracts total RNA from cells or tissues. It can maintain the integrity of RNA when breaking and dissolving cells. After adding chloroform and centrifuging, the sample was divided into a water sample layer and an organic layer. RNA exists in the aqueous layer. After collecting the water sample layer above, RNA can be recovered by isopropanol precipitation.

This method has good separation effects on small and large amounts of tissues and cells, whether they are human, animal, plant, or bacterial tissues. The simplicity of TRzol reagent operation allows for the simultaneous processing of multiple samples. All operations can be completed within one hour. The total RNA extracted by TRzol can avoid contamination of DNA and proteins. Therefore, it can perform RNA blot analysis, spot hybridization, poly (A)+selection, in vitro transcription, RNAse protection analysis, and molecular cloning.

TRzol reagent can promote the precipitation of various RNAs of different species and molecular weights. For example, RNA extracted from rat liver was electrophoretic on agarose gel and stained with ethidium bromide, and many discontinuous high molecular weight bands (mRNA and hnRNA components) between 7 kb and 15 kb, two dominant ribosomes~5kb (28S) and~2kb (18S), and low molecular weight RNA between 0.1 and 0.3 kb (tRNA, 5S) were seen. When the extracted RNA is diluted with TE, its A 260/A 280 ratio is ≥ 1.8.

product storageTRzol can be stored stably for 12 months at room temperature. However, to achieve the desired effect, we recommend storing it in an environment of 2-8 ℃. (For specific instructions, please refer to the manual)

The products currently being sold by the company: