Automatic detection instrument for pine wilt disease

　　Automatic detection instrument for pine wilt diseaseIn order to maximize the effectiveness of the "invisible guardian", three core points need to be grasped during the use process: precise positioning of sample collection, selecting the xylem at the junction of healthy and suspected lesions in the middle and lower parts of pine tree trunks, collecting 3-5 samples from each tree, avoiding rotten and insect infested areas, and ensuring sample representativeness; Sample processing requires standardized operations, releasing nematodes through grinding and using specialized extraction reagents to remove impurities such as lignin and humic acid, in order to avoid interfering with the detection results; Equipment maintenance needs to be carried out regularly, and portable instruments need to be calibrated 1-2 times a year, with a focus on checking detection sensitivity and battery life; Laboratory equipment requires regular maintenance of optical modules and temperature control systems to ensure stable detection accuracy.

1Automatic detection instrument for pine wilt diseaseInstrument usage

The rapid detection system for pine wood nematodes mainly includes processes such as isolation, DNA extraction, and fluorescence quantitative PCR detection. It can quickly complete the detection of pine wood nematodes, automatically display the detection results and print the detection report, without the need for manual interpretation, with an accuracy rate of over 99%, greatly improving the efficiency of pine wood nematode detection. The field rapid detection system is equipped with a portable and mobile operation, providing convenience for the detection of pine wood nematodes in the field. The automated molecular detection system for pine wood nematodes is widely used in forestry, forest defense stations, quarantine, customs, universities, scientific research institutions and other units.

2、 Instrument features

1. The system has high detection accuracy.

2. The intelligent pine wood nematode DNA rapid detection system is equipped with an automated detection program for pine wood nematode DNA, which does not require complicated program settings and can run automatically with one click. It can not only detect nematode separation solution, but also directly detect pine wood chips without separating nematodes.

3. The system detection task is easy to operate, time-saving and efficient, and suitable for various forestry and forest defense departments. It only takes 60 minutes to complete the entire process including sample processing, DNA extraction, computer-based testing, and result interpretation.

4. By using amplification fluorescence technology, laboratory testing and identification requirements can be completed through the system host.

3、 Instrument features

1. Small size, light weight, and easy to carry. Easily meet the needs of outdoor experiments.

2. Built in 10 inch high-definition capacitive screen PDA, touch screen operation, simple and fast.

3. Marlow high-quality Peltier refrigeration chips, combined with German PT1000 temperature sensor and temperature control mode with electric resistance heating compensation edge, greatly shorten the experimental time.

4. The whole board adopts a 3-second fast lighting mode to ensure the consistency of the hole positions in the experimental results.

5. Simple and intuitive software guidance, making it easy to start detection experiments.

4、 Technical parameters

Sample capacity: 48x0.2ml, supports 8-tube connection

Applicable consumables: Common transparent PCR consumables, 8x0.2ml tube, 0.2ml single tube

Reaction system: 5-120ul reaction mode system

Heating/cooling module: high-performance imported semiconductor thermoelectric module

Temperature control range: 4 ° C-99 ℃

Hot cover temperature: 30 ℃ -108 ℃ (default 105 ℃, hot cover temperature adjustable)

Heating rate: 3.5 ℃/s (MAX)

Cooling rate: 2.5 ℃/s (MAX)

Temperature control accuracy: ± 0.1 ° C

Temperature uniformity: ± 0.25 ° C