Carp Epithelial Tumor Cell Line (EPC)

Product Name:Carp Epithelial Tumor Cell Line (EPC)

Product Category: Other Cell Lines

Growth characteristics: Wall attached growth

Cultivation system: MEM+10% FBS, cultured at 28 ° or 21 ° C

Passage method: 1:2 passage

Cell morphology: Epithelial like

Specification: 1 × 10 ^ 6 cells/T25 culture bottle

Item number: CS-C2008

STR -
isozyme
chromosome
Use permission Class A
Carp Epithelial Tumor Cell Line (EPC)Experimental methods and steps
（1） The pre-treatment involves weighing frogs or toads on a scale, and then injecting colchicine intraperitoneally at a dose of 10 micrograms per gram of animal body weight.
（2） After 4 hours of sampling and injection, the animals were euthanized, and the skin and muscles were cut open with scissors. The thigh bone and tibia were removed, and the muscles on the femur were cleaned. Then, a small piece of gauze was rubbed back and forth to clean. Cut open both ends of the femur and slowly wash the bone marrow cells into a centrifuge tube using a syringe (containing 5ml of 1% sodium citrate solution). After equilibration, centrifuge for 8 minutes at a speed of 1000 revolutions per minute.
（3） Remove the supernatant by low permeability suction, leave 0.2ml of precipitate, add 0.046M KCL solution to the mark of 8ml, rinse the precipitate with a pipette, and treat in a constant temperature water bath (28 ℃) for 20 minutes or longer.
（4） After fixing the low permeability, centrifuge at a speed of 1000 revolutions per minute for 10 minutes. Carefully aspirate the supernatant with a straw, leaving 0.2ml of sediment. After dispersion, slowly add 6ml of fixative along the tube wall. After mixing, let it stand still and fix for 20 minutes. Repeat the fixation 1-2 times, centrifuge at the same speed and time as described above.
（5） Suck off the supernatant with drops, leave 0.2ml of sediment, add 4 drops of newly prepared fixative, and mix the sediment evenly. Take out pre frozen slides from the refrigerator, drop 3 drops of cell suspension onto each slide, immediately blow air onto the slides with your mouth to evenly disperse the cells, and bake them back and forth on an alcohol lamp flame to help the chromosomes disperse and unfold better. （6） Stain with a 10:1 Giemsa staining solution for 20 minutes (Giemsa stock solution diluted with pH=6.8 phosphate buffer), then rinse with water, dry the slides, and examine under a microscope. （7） Microscopic examination is used to locate the dispersed metaphase chromosomes under a medium magnification microscope, and then transferred to a high magnification microscope to observe in detail the number of chromosomes in amphibians, including the number of large, medium, and small chromosomes.
Notes:
After receiving the cells, if you find that the dry ice has evaporated completely, the cap of the cryovial has fallen off, is damaged, or the cells are contaminated, please contact us immediately.
2. Do not open the bottle cap when receiving the cells. Wipe the bottle with alcohol and place it in the incubator for 2-4 hours (depending on the cell density) to stabilize the cell state. Next, observe the cell growth under an inverted microscope and take photos of the cells at different magnifications (it is recommended to take a photo of the overall appearance when collecting the cells, observe the color of the culture medium and whether there is any leakage, and then take a photo of the cell status under the microscope, one at 100 * and one at 200 *). Observe and record whether there is any contamination during the transportation of the cells. As a basis for our sales.
3. Due to various factors such as environment, operation, and transportation affecting cell status, our company only guarantees the cell status of customers within one week after receiving the cells. Therefore, when customers need after-sales service, they need to provide proof of the time of receiving the cells and proof of the time of receiving the goods and communication with customer service personnel after discovering the problem. The interval between periods cannot exceed 7 days.
4. All animal cells are considered to have potential biological hazards and must be operated in a Level 2 biosafety platform. Please pay attention to protection and all waste liquids and organs that have come into contact with these cellsCarp Epithelial Tumor Cell Line (EPC)The dish needs to be sterilized before it can be discarded.
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