ForteBio Octet RED96 Interaction Analyzer Instrument

Key Features:

• High throughput technology for simultaneous detection of eight channels;

• Real time analysis without the need for labeling;

• High sensitivity detection results;

• Characterization of proteins and antibodies;

• Screening and analysis of small molecule compounds and molecular fragments;

• Fiber optic biosensors are inexpensive, recyclable, and reusable;

• Simple and easy-to-use 'dip test and read' method;

• The sample does not need to be pureIt can directly detect crude biological samples such as serum, plasma, tissue lysate, and cell lysate.

Application scope:

Concentration analysis

adoptOctet96The simple and easy-to-use 'dip and read' method of the system allows users to measure the concentration of natural proteins and other biomolecules using the results of direct intermolecular binding96Orifice plate, concentration measurement can be done in one step. The dynamic range of concentration measurement is wide, covering millions of times the magnitude difference, and concentrations ranging from the lower limit of nanograms per milliliter to the upper limit of milligrams per milliliter can be used. By using eight channels for simultaneous detection, you can achieve this in just a short amount of time32Get it within minutes96The result of a sample.Octet RED96The system has added a reset function for biosensors, allowing the same biosensor to be reused multiple times after regeneration. High sensitivity double antibody sandwich enzyme-linked immunosorbent assay or other ligand binding immunoassay can be performed on this system in just one or two hours! All experiments are fully automated and once started, they are automatically completed according to the program without the need for additional human intervention.

kinetic analysis

Octet RED96The system can be used to measure interactions between proteins and biomolecules, including interactions between proteins and small molecules. It can provide binding rate constants including（Ka）Dissociation rate constant（Kd）Harmony and Constant（KD）Reliable dynamic information inside. Using eight channels for simultaneous measurement,Octet RED96The system can provide you with8×12Multi dimensional analysis enables you to automatically analyze and measure up to960An interaction. You can use it for rapid antibody screening and antigen epitope analysis identification in the development of biopharmaceuticals. If you are conducting traditional small molecule drug development, simply8You can use it to complete tasks beyond the hour400Screening of small molecule compounds. After filtering, you can still use the sameOctet RED96The system accurately determines the kinetic properties of candidate drugs.

Simplified workflow

Octet RED96The system is a fully automated, mature, and reliable testing platform. It has data collection and analysis software, which is simple, intuitive, and powerful.Octet RED96The system can recycle biosensors for reuse after regeneration, allowing you to optimize workflow, reduce costs, and improve efficiency.

Technical specifications:

Analytical technique: biofilm interference technologyBiolayer Interferometry(BLI)

Flux: Up to eight tests can be conducted simultaneously, each96Orifice plates can be used for up to96Item testing

Biosensor type: Fiber optic biosensors, which can be used once or reused after two generations

Sample analysis time: for individualslgGQuantitative analysis,8Only one sample is needed2minute,96No more than one sample32minute. Actual measurements are used for dynamic analysis, with a wide range of experimental time allowed5Minutes to arrive4The hours vary.

Information provided: Dynamics and affinity analysis, concentration determination, automatic calculation of concentration

personlgGQuantitative analysis scope:200rpmAt the rotational speed,0.5microgram/Milliliters to2000microgram/milliliter.1000rpmAt the rotational speed,0.05microgram/Milliliters to100microgram/milliliter.

Data display: Multiple charts display the actual values, fitted values, and residual values of dynamic measurements, providing a comprehensive dynamic data table and data graph for one click automatic concentration calculation. The results include standard curve graphs and calculated concentration values for unknown samples

Baseline noise: ≤3pm（RMS）

Size:47*43*53Centimeters (height)*deep*Wide)

Weight:28.6kilogram

Circuit requirements: AC100-240Fu,5.0-2.0Ampere,50/60Hertz, single-phase. Power:120Wa (the most abundant)240Wa)

Safety standards: Compliant with the European Community（CE）The Canadian Standards Institute（CSA）Standard

Sample types: including proteins, antibodies, peptides, culture media containing serum, and media containingDMSOA wide range of sample types, including buffer solutions, interstitial fractions, clear solutions from untreated cell cultures, crude cell lysates, etc

Sample format: Standard black flat bottom96orifice plate

Sample volume: per well180-220Microliter non-destructive testing

Circular oscillator speed range:100-1500rpm, can also be * stationary

Test temperature: above ambient temperature4℃ to40Between ℃,1℃ increasing gradually

ForteBio Octet RED96The system only requires a small amount of samples without labeling, and can provide real-time analysis results of biomolecule interactions. It adopts eight channels for simultaneous detection, with each channel having its own independent detector, high sensitivity of measurement results, and fast and convenient measurement process. On the one hand, it can be used for rapid measurement of protein and other biomolecule concentrations, and on the other hand, it can be used for affinity and kinetic measurements, as well as for studying protein-protein interactions and protein dynamics-The ideal choice for small molecule interactions.Octet RED96The system can be widely used for concentration measurement of antibodies and proteins, research and development of biological processing technology, quality analysis, antibody screening, cell line optimization, antigen epitope identification, antibody pairing, ligand binding, screening and analysis of small molecules and molecular fragments, study of intercellular signal transduction mechanisms, and monitoring of infectious diseases. As a leading research instrument platform,Octet RED96System performance *, demonstrated in protein analysis. Only using this instrument and equipment can replace high-performance liquid chromatography, enzyme-linked immunosorbent assay, and other microfluidics-SPRMultiple facilities, including instruments and equipment, accelerate and simplify your scientific research and production.