Human soluble CD100 (sCD100) ELISA kit

Human soluble CD100 (sCD100) ELISA kit

✦ Productsinformation

【purpose】

This reagent kit usesOne step sandwich enzyme-linked immunosorbent assay (ELISA) for quantitative detection of serum, plasma, tissue, cells or other related biological fluids in vitrosCD100Concentration.

【Detection Principle】

Coat the target antibody withIn a 96 well microplate, a solid phase carrier is prepared and standard samples or specimens are added to the micropores. The target is bound to the antibody attached to the solid phase carrier, and then horseradish peroxidase labeled antibody is added. The unbound antibody is washed and washed again before adding TMB substrate for color development. TMB is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. The depth of color is positively correlated with the target in the sample. Measure the absorbance (O.D. value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader and calculate the sample concentration.

【specificity】

detectableMeasure sCD100 in the sample, andThere is no significant cross reactivity with other similar substances.

【repetitiveness】

Both intra plate and inter plate coefficients of variation<10%

【Within batch difference】

Take the same batch of reagent kits for quantitative testing of low, medium, and high value samples, and measure each sample continuouslyCalculate the average and SD values of samples with different concentrations 20 times.

【Batch difference】

selectThree different batches of reagent kits were used to quantitatively measure low, medium, and high value samples. Each sample was tested 8 times using the same reagent kit, and the average and SD values of samples with different concentrations were calculated.

【precision】

Coefficient of variation of precision measured values using samplesCV represents. CV (%)=SD/mean × 100 CV<10% Inter batch difference: CV<13%

【stability】

After testing, the reagent kit was stored at the recommended temperature within its validity period, and its activity reduction rate was less than5%. To minimize the impact of external factors on the detection values before and after kit damage, the laboratory environment should be kept as consistent as possible, especially the temperature, humidity, and incubation conditions inside the laboratory. Secondly, having the same experimenter perform the operation can reduce human error.

Human soluble CD100 (sCD100) ELISA kit

✦ Self provided items required for the experiment

1. 450 ± 10nm filter enzyme-linked immunosorbent assay (recommended to preheat the instrument before use)

2. High precision sampler and nozzle: 0.5-10uL, 2-20uL, 20-200uL, 200-1000uL

3. Eppendorf pipette

4. Distilled water or deionized water

5. Defatted cotton absorbent paper

6. 37 ℃ constant temperature box

7. 100ml and 1000ml measuring cylinders