Mouse Toll like Receptor 9 (TLR-9/CD28) ELISA Kit

Mouse Toll like Receptor 9 (TLR-9/CD28) ELISA KitDetection Principle

Coat the target antibody in a 96 well microplate to form a solid phase carrier. Add standard or specimen to each well, and the target is bound to the antibody on the solid phase carrier. Then add horseradish peroxidase labeled antibody. Wash the unbound antibody and wash it again before adding TMB substrate for color development. TMB is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. The depth of color is positively correlated with the target in the sample. Measure the absorbance (O.D. value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader and calculate the sample concentration.

▎ repetitiveness

Both intra plate and inter plate coefficients of variation<10%.

▎ Within batch difference

Quantitatively detect low, medium, and high value samples using the same batch of reagent kits. Each sample is measured continuously for 20 times, and the average and SD values of samples with different concentrations are calculated separately.

▎ batch-to-batch variation

Select 3 different batches of reagent kits to quantitatively measure low, medium, and high value samples. Repeat the measurement 8 times for each sample using the same reagent kit, and calculate the average and SD values of samples with different concentrations

precision

Precision is represented by the coefficient of variation (CV) of the measured values of the sample. CV (%)=SD/mean × 100 CV<10% Inter batch difference: CV<13%

stability

After testing, the reagent kit was stored at the recommended temperature within its validity period, and its activity reduction rate was less than 5%.
To reduce the influence of external factors on the detection values before and after the destruction of the reagent kit, the laboratory environment conditions should be kept as consistent as possible, especially the temperature, humidity, and incubation conditions inside the laboratory. Secondly, having the same experimenter perform the operation can reduce human error.

In order to help you better solve various problems that may arise in the experiment, we have designed these problem guides, along with the instructions in the reagent kit, for your reference and use. Many factors can lead to the failure of immunoassay experiments, and most technical errors can be avoided by thoroughly reading and accurately understanding the instructions and experimental steps. If you have any comments or suggestions regarding the instructions inside the reagent kit, please feel free to provide feedback. We look forward to hearing your voice and improving our products to better serve you.
Carefully read the instruction manual
Check the expiration date on the label of the reagent kit. If it exceeds the expiration date, please do not use it.
Confirm that all reagents are complete (quantity, volume) according to the instructions
◆ Specimen preparation should be standardized, and the volume of each specimen should be in accordance with2-3Prepare (store) quantities with multiple wells or more, and try to pack them as backups.
Unable to conduct experiments in the short term, pay attention to low-temperature storage
Prepare all necessary additional items for the experiment, such as pipettes, test tubes, cleaners, and enzyme-linked immunosorbent assay (ELISA) analyzers
◆ Balance the reagents used according to the instructions to room temperature, and determine the required amount of reagents based on the number of test specimens
Check the storage conditions of each reagent in the kit to ensure that all reagents are stored according to the recommended conditions in the kit instructions.
Check for unstable or deteriorated reagent solutions (such as precipitation or discoloration). Some reagents, such as standard dilutions or test dilutions, may contain precipitates in their design. All reagents must be thoroughly mixed before being dropped into the enzyme-linked immunosorbent assay (ELISA) plate. Due to the characteristics of the precipitate, it is necessary to stir continuously before adding it to the enzyme-linked immunosorbent assay (ELISA) plate.
Ensure sufficient incubation time and temperature.
Do not replace or mix existing reagents with reagents of different production batches.
◆ Avoid foam when mixing or dissolving protein solution.
Arrange the experimental process before starting the experiment.
Clean the workbench before the experiment begins.
If there are any problems, you should contact our company or agentCommunicate.