Pine wood nematode automatic detector

　　Pine wood nematode automatic detectorThe core value lies in transforming complex detection technologies into practical tools that can be implemented at the grassroots level. The Federal Micrometer developed by the Chinese Academy of Forestry is only the size of a mobile phone and weighs less than 300 grams. Grassroots forestry personnel can carry it deep into deep mountain forests. The operation process is simplified to three steps: take 1 gram of suspicious branches, cut them into pieces and soak them, process them with an oscillator for 5 minutes, then add them to the detection card box. After 20 minutes, the results can be visually judged through the "two red bars" with an accuracy rate of 100%, without the need for a professional laboratory background. Behind this "foolproof" design is a breakthrough in recombinant enzyme polymerase amplification (RPA) technology, which can specifically amplify nematode genes at room temperature, increasing detection efficiency by more than 10 times compared to traditional methods.

1Pine wood nematode automatic detectorInstrument usage

The rapid detection system for pine wood nematodes mainly includes processes such as isolation, DNA extraction, and fluorescence quantitative PCR detection. It can quickly complete the detection of pine wood nematodes, automatically display the detection results and print the detection report, without the need for manual interpretation, with an accuracy rate of over 99%, greatly improving the efficiency of pine wood nematode detection. The field rapid detection system is equipped with a portable and mobile operation, providing convenience for the detection of pine wood nematodes in the field. The automated molecular detection system for pine wood nematodes is widely used in forestry, forest defense stations, quarantine, customs, universities, scientific research institutions and other units.

2、 Instrument features

1. The system has high detection accuracy.

2. The intelligent pine wood nematode DNA rapid detection system is equipped with an automated detection program for pine wood nematode DNA, which does not require complicated program settings and can run automatically with one click. It can not only detect nematode separation solution, but also directly detect pine wood chips without separating nematodes.

3. The system detection task is easy to operate, time-saving and efficient, and suitable for various forestry and forest defense departments. It only takes 60 minutes to complete the entire process including sample processing, DNA extraction, computer-based testing, and result interpretation.

4. By using amplification fluorescence technology, laboratory testing and identification requirements can be completed through the system host.

3、 Instrument features

1. Small size, light weight, and easy to carry. Easily meet the needs of outdoor experiments.

2. Built in 10 inch high-definition capacitive screen PDA, touch screen operation, simple and fast.

3. Marlow high-quality Peltier refrigeration chips, combined with German PT1000 temperature sensor and temperature control mode with electric resistance heating compensation edge, greatly shorten the experimental time.

4. The whole board adopts a 3-second fast lighting mode to ensure the consistency of the hole positions in the experimental results.

5. Simple and intuitive software guidance, making it easy to start detection experiments.

4、 Technical parameters

Sample capacity: 48x0.2ml, supports 8-tube connection

Applicable consumables: Common transparent PCR consumables, 8x0.2ml tube, 0.2ml single tube

Reaction system: 5-120ul reaction mode system

Heating/cooling module: high-performance imported semiconductor thermoelectric module

Temperature control range: 4 ° C-99 ℃

Hot cover temperature: 30 ℃ -108 ℃ (default 105 ℃, hot cover temperature adjustable)

Heating rate: 3.5 ℃/s (MAX)

Cooling rate: 2.5 ℃/s (MAX)

Temperature control accuracy: ± 0.1 ° C

Temperature uniformity: ± 0.25 ° C