Pine wood nematode rapid detection instrument

　　Pine wood nematode rapid detection instrumentThe forward movement of the prevention and control strategy promotes the penetration of equipment demand into diversified scenarios: at the field monitoring end, UAVs are equipped with LAMP detection modules to achieve large-scale screening of epidemic areas. Mount Huangshan Scenic Spot in Anhui Province has shortened the time of epidemic discovery from one year to three months through this technology, reducing the prevention and control cost by 60%; In the early diagnosis stage, immunological detection equipment can identify specific antibody proteins in the early stage of nematode infection, achieving "asymptomatic warning". Guangdong Ruyang Forest Farm has used this technology to rescue early infected pine trees; At the circulation quarantine end, the portable PCR equipment deployed at the port can complete the screening of imported timber in 30 minutes. In 2023, a coastal port intercepted 3 batches of positive timber based on this, avoiding losses exceeding 50 million yuan.

1Pine wood nematode rapid detection instrumentInstrument usage

The rapid detection system for pine wood nematodes mainly includes processes such as isolation, DNA extraction, and fluorescence quantitative PCR detection. It can quickly complete the detection of pine wood nematodes, automatically display the detection results and print the detection report, without the need for manual interpretation, with an accuracy rate of over 99%, greatly improving the efficiency of pine wood nematode detection. The field rapid detection system is equipped with a portable and mobile operation, providing convenience for the detection of pine wood nematodes in the field. The automated molecular detection system for pine wood nematodes is widely used in forestry, forest defense stations, quarantine, customs, universities, scientific research institutions and other units.

2、 Instrument features

1. The system has high detection accuracy.

2. The intelligent pine wood nematode DNA rapid detection system is equipped with an automated detection program for pine wood nematode DNA, which does not require complicated program settings and can run automatically with one click. It can not only detect nematode separation solution, but also directly detect pine wood chips without separating nematodes.

3. The system detection task is easy to operate, time-saving and efficient, and suitable for various forestry and forest defense departments. It only takes 60 minutes to complete the entire process including sample processing, DNA extraction, computer-based testing, and result interpretation.

4. By using amplification fluorescence technology, laboratory testing and identification requirements can be completed through the system host.

3、 Instrument features

1. Small size, light weight, and easy to carry. Easily meet the needs of outdoor experiments.

2. Built in 10 inch high-definition capacitive screen PDA, touch screen operation, simple and fast.

3. Marlow high-quality Peltier refrigeration chips, combined with German PT1000 temperature sensor and temperature control mode with electric resistance heating compensation edge, greatly shorten the experimental time.

4. The whole board adopts a 3-second fast lighting mode to ensure the consistency of the hole positions in the experimental results.

5. Simple and intuitive software guidance, making it easy to start detection experiments.

4、 Technical parameters

Sample capacity: 48x0.2ml, supports 8-tube connection

Applicable consumables: Common transparent PCR consumables, 8x0.2ml tube, 0.2ml single tube

Reaction system: 5-120ul reaction mode system

Heating/cooling module: high-performance imported semiconductor thermoelectric module

Temperature control range: 4 ° C-99 ℃

Hot cover temperature: 30 ℃ -108 ℃ (default 105 ℃, hot cover temperature adjustable)

Heating rate: 3.5 ℃/s (MAX)

Cooling rate: 2.5 ℃/s (MAX)

Temperature control accuracy: ± 0.1 ° C

Temperature uniformity: ± 0.25 ° C