Rat IL-12 ELISA Kit

Product Name: Rat Interleukin12 (IL-12) ELISA Kit

Product background (for reference only)：interleukin-Interleukin-12 (IL-12) is an important pro-inflammatory cytokine, mainly composed ofDendritic cells（DCs）、 Antigen presenting cells (APCs) such as macrophages and B cells are produced. It plays a crucial role in both innate and adaptive immunity, particularly in Th1 type immune responses and anti-tumor immunity.

Product Specifications48T，96T

Detection scope:Please refer to the instruction manual.

Sensitivity:most-The low detection concentration is less than 0.1.

Specificity:Do not cross react with other soluble structural analogues.

Repeatability:The coefficient of variation within the board is less than10%, with an inter plate coefficient of variation of less than 15%.

Sample type:serumPlasma and other biological fluids.

Storage temperature:Refrigerate and store at 2-8 ℃.

1、 Kit composition:

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2、 Operation steps:

1. Balance from room temperature20minTake out the required Flat noodles from the rear aluminum foil bag, and seal the remaining Flat noodles with a self sealing bag4℃。

2. Set up standard wells and sample wells, and add different concentrations of standard samples to each standard well50ML.

3. Add the sample to be tested into the sample well50MLBlank holes are not added.

4. Add horseradish peroxidase to each well of the standard and sample wells, except for blank wells（HRP）Labeled detection antibodies100MLSeal the reaction hole with a sealing film,37Incubate in a ℃ water bath or constant temperature box60min.

5. Discard the liquid, pat dry on absorbent paper, and fill each hole with detergent（350ML）Let it stand still1minDiscard the detergent, pat dry on absorbent paper, and repeat the washing process5The board can also be washed using a board washing machine.

6. Add substrate to each wellATheBeach50ML，37Incubate at ℃ in the dark15min.

7. Add termination solution to each well50ML，15minInside, within450nmMeasure the wavelength of each holeODValue.

3、 Use safety measures:

Please be sure to wear laboratory clothes, protective face masks, and laboratory gloves to ensure proper laboratory protection. Direct contact with the product is strictly prohibited. Please do not dispose of the remaining products at will. Please follow the laboratory's relevant regulations for the disposal of biological and chemical waste.

4、 Technical support and after-sales service:

If you encounter any problems during use, please feel free to contact our technical support team at any time. We offer7×24Hourly consultation service ensures that you have no worries during use.

5、 Contact us:

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Rat interleukin12 (IL-12) ELISA Kit