Rat complement fragment 3d (C3d) ELISA kit

NegotiableUpdate on 04/25

Model

Nature of the Manufacturer: Producers

Product Category

Place of Origin

Online Consult

Overview

Rat complement fragment 3d (C3d) ELISA kit: complement fragment #160; C3d #160; It is during the activation process of the complement system; #160; C3 #160; The key degradation products of proteins, as "bridge molecules" connecting innate immunity and adaptive immunity, play important roles in immune regulation, memory B cell activation, and autoimmune diseases.

Product Details

Product Name: Rat Complement Fragment3D (C3d) ELISA Kit

Product background (for reference only)：Complement fragmentC3d is a key degradation product of C3 protein during the activation of the complement system. As a "bridge molecule" connecting innate immunity and adaptive immunity, it plays an important role in immune regulation, memory B cell activation, and autoimmune diseases.

Product Specifications48T，96T

Detection scope:Please refer to the instruction manual.

Sensitivity:most-The low detection concentration is less than 0.1.

Specificity:Do not cross react with other soluble structural analogues.

Repeatability:The coefficient of variation within the board is less than10%, with an inter plate coefficient of variation of less than 15%.

Sample type:serumPlasma and other biological fluids.

Storage temperature:Refrigerate and store at 2-8 ℃.

1、 Kit composition:

Kit components (96T)	Standard product（6 pipes)	0.5ml/pipe*6管（液体）
	Pre packaged items have been reflected	12Kong*8article 1block
	HRP labeled antibody	1bottle(6mL)
	Concentrated washing solution	1bottle(30mL)
	Termination liquid	1bottle(6mL)
	substrate solutionA	1bottle(6mL)
	substrate solutionB	1bottle(6mL)

Free proxy testing service, helping scientific research worry free!

In scientific research experiments,ELISAThe selection and use of reagent kits are crucial for experimental results. To help you complete experiments more efficiently and accurately, we have specially launched“ELISAFree testing service for reagent kitsSo you don't have to worry about experimental operations and data analysis, focus on scientific research and innovation!

The advantages of free testing services:

1、Time and effort saving: You only need to provide samples, and we will complete the experimental operation and data analysis for you, greatly reducing the experimental cycle and freeing up your research energy.

2、Accurate and reliable: The entire experiment was conducted in compliance withGLPConducted in a standard laboratory, operated by an experienced technical team to ensure the accuracy and reproducibility of experimental results.

3、Personalized customization: supports single sample multi index detection, high-throughput screening of large samples, optimizes experimental plans for special samples (such as low abundance targets and complex matrices), ensuring data accuracy and traceability.

4、Intelligent report: We provide raw data, standard curve graphs, and statistical analysis, support multi-dimensional data visualization, and can combine your needs for in-depth bioinformatics interpretation, providing solid support for research conclusions.

2、 Operation steps:

1. Balance from room temperature20minTake out the required Flat noodles from the rear aluminum foil bag, and seal the remaining Flat noodles with a self sealing bag4℃。

2. Set up standard wells and sample wells, and add different concentrations of standard samples to each standard well50ML.

3. Add the sample to be tested into the sample well50MLBlank holes are not added.

4. Add horseradish peroxidase to each well of the standard and sample wells, except for blank wells（HRP）Labeled detection antibodies100MLSeal the reaction hole with a sealing film,37Incubate in a ℃ water bath or constant temperature box60min.

5. Discard the liquid, pat dry on absorbent paper, and fill each hole with detergent（350ML）Let it stand still1minDiscard the detergent, pat dry on absorbent paper, and repeat the washing process5The board can also be washed using a board washing machine.

6. Add substrate to each wellATheBeach50ML，37Incubate at ℃ in the dark15min.

7. Add termination solution to each well50ML，15minInside, within450nmMeasure the wavelength of each holeODValue.

3、 Use safety measures:

Please be sure to wear laboratory clothes, protective face masks, and laboratory gloves to ensure proper laboratory protection. Direct contact with the product is strictly prohibited. Please do not dispose of the remaining products at will. Please follow the laboratory's relevant regulations for the disposal of biological and chemical waste.

4、 Technical support and after-sales service:

If you encounter any problems during use, please feel free to contact our technical support team at any time. We offer7×24Hourly consultation service ensures that you have no worries during use.

5、 Contact us:

If you are interested in ourELISAIf you are interested in reagent kits or free testing services, please feel free to contact our website customer service at any time to obtain exclusive solutions and start an easy and efficient scientific research experience!

Choose usELISAReagent kit, let professionals do professional things! We will all safeguard your scientific research journey with a rigorous attitude, efficient execution, and reliable results!

Rat complement fragment3D (C3d) ELISA Kit