Rat muscarinic acetylcholine receptor assay M-AChR kit

Shanghai Fantai BiotechnologyTechnology Co., Ltd. is a high-tech biological enterprise engaged in the research and development, production, and sales of biological reagents and test kits.

Rat muscarinic acetylcholine receptor assay M-AChR kit

calculate
Using the concentration of the standard substance as the horizontal axis,The OD value is plotted on the vertical axis, and a standard curve is drawn on the coordinate paper. Based on the OD value of the sample, the corresponding concentration is determined from the standard curve; Multiply by the dilution factor again; Alternatively, the linear regression equation of the standard curve can be calculated using the concentration and OD value of the standard substance. By substituting the OD value of the sample into the equation, the sample concentration can be calculated, and then multiplied by the dilution factor to obtain the actual concentration of the sample.
Precautions
1. The kit should be balanced at room temperature for 15-30 minutes before use when taken out from the cold storage environment. If the enzyme coated plate is not used up after opening, the Flat noodles should be stored in a sealed bag.
2. There may be crystal precipitation in concentrated washing solution. When diluting, it can be dissolved by heating in a water bath, and washing does not affect the results.
3. Sample dispensers should be used for each step of sample addition, and their accuracy should be regularly checked to avoid experimental errors. The sample addition time should be controlled within 5 minutes. If there are a large number of specimens, it is recommended to use a sampling gun for sample addition.
4. Please make a standard curve and perform a double hole at the same time as each measurement. If the content of the substance to be tested in the specimen is too high (the OD value of the sample is greater than the OD value of the standard well * well), please dilute the sample diluent by a certain factor (n times) before measuring. When calculating, please multiply * by the total dilution factor (x n x 5).
5. The sealing film is only for one-time use to avoid cross contamination.
6. Please store the substrate away from light.
7. Strictly follow the instructions for operation, and the judgment of the test results must be based on the reading of the enzyme-linked immunosorbent assay reader.
8. All samples, detergents, and various waste materials should be treated as infectious agents.
9. Components from different batch numbers of this reagent must not be mixed.
10. If there is any discrepancy with the English manual, the English manual shall prevail.Fantai Biological Anti signal Recognition Particle Antibody ELISA Detection SRP KitStorage conditions and expiration date
1. Storage of reagent kit: 2-8 ℃.
2. Validity period: 6 months

Advantages of company products:

1. Full: The company provides tens of thousands of products, including biological reagents, ELISA kits, standard products, culture media, original consumables, etc. Basically, various scientific research products can be found in our company.

2. New: The product update speed is fast, with new products appearing almost every week.

3. Excellent: Good product quality, *.

4. Multiple Brands: The company represents imported reagent brands such as Amresco, Sigma, Fluka, Alfa, TCI, Merck, etc. Domestic brands have better prices, and we supply high-quality pure, analytical pure, chemical pure, reagent grade, benchmark reagent, experimental pure, teaching reagent, high-purity reagent, chromatographic pure, spectral pure, electronic pure and other reagent grades (can be customized according to customer needs).

5. After sales: Our company has a high-quality technical team, and once the product is sold,The product is only for scientific research purposesIf encountering difficulties during the experiment, online technical consultation can be provided. Make sure you have no worries when using the product.

Sample processing and requirements:

1. Serum: Whole blood samples should be left at room temperature for 2 hours or overnight at 4 ℃, then centrifuged at 1000g for 20 minutes to obtain the supernatant for detection. Alternatively, the samples can be stored at -20 ℃ or -80 ℃, but repeated freezing and thawing should be avoided.

2. Plasma: EDTA or heparin can be used as anticoagulants. Within 30 minutes after specimen collection, centrifuge at 2-8 ° C 1000g for 20 minutes, or store the specimen at -20 ° C or -80 ° C, but avoid repeated freezing and thawing.

3. Tissue homogenate: Rinse the tissue with pre cooled PBS (0.01M, pH=7.4) to remove residual blood (lysed red blood cells in the homogenate can affect measurement results), weigh and crush. Mix the shredded tissue with the corresponding volume of PBS (usually in a weight to volume ratio of 1:9, for example, 1g of tissue sample corresponds to 9mL of PBS, the specific volume can be adjusted appropriately according to experimental needs, and records should be kept. Recommend adding protease inhibitors to PBS and grinding thoroughly on ice in a glass homogenizer. To further lyse tissue cells, the homogenate can be sonicated or subjected to repeated freeze-thaw cycles. Centrifuge the homogenate at 5000 × g for 5-10 minutes, and take the supernatant for detection.

4. Cell culture supernatant or other biological specimens: Centrifuge 1000g for 20 minutes, take the supernatant for detection, or store the specimen at -20 ℃ or -80 ℃, but avoid repeated freezing and thawing.

Note: Hemolysis of specimens can affect the test results, therefore hemolysis specimens are not suitable for this test.

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