Recombinant bovine trypsin ELISA kit

1、 Parameter specifications

:Recombinant bovine trypsin ELISA kit

Item number: E-53011

【 Price 】: 2400

【 Supply period 】: 1-3 days (spot supply)

: Qualitative and Quantitative Analysis of Tested Samples

: 96T/48T (two specifications)

【 Detection Method 】: Enzyme Immunoassay/Enzyme Linked Immunoassay (ELISA)

【 Storage conditions 】: 2-8 ℃

: Liquid boxed packaging

: Industry price advantage, reliable quality, and guaranteed replacement and refund in case of objective quality issues

【 Sample to be tested 】: body fluids, serum, plasma, cell culture supernatant, urine, tissue homogenate, atrial water standard, etc

2、 Specimen requirements

1. Serum: After natural coagulation of warm blood for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 revolutions per minute). Carefully collect the supernatant. If precipitation forms during storage, it should be centrifuged again.

2. Plasma: Select EDTA, sodium citrate or other anticoagulants according to the requirements of the specimen, mix for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant. If precipitation forms during storage, it should be centrifuged again.

3. Urine: collected in sterile tubes. Centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant. If precipitation forms during storage, it should be centrifuged again. Follow this procedure for pleural effusion, ascites, and cerebrospinal fluid.

4. Cell culture supernatant: When detecting secreted components, collect them using sterile tubes. Centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant.

5. Tissue specimen: After cutting the specimen, weigh it. Add a certain amount of PBS, pH 7.4. Quickly freeze and store in liquid nitrogen for future use. The specimen remains at a temperature of 2-8 ℃ even after melting. Add a certain amount of PBS (pH 7.4) or tissue protein extraction reagent, and homogenize the specimen manually or with a homogenizer. Centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant. After packaging, one portion is to be tested, and the rest is to be frozen for future use.

3、 Question and Answer

Analysis of Negative and Positive Control of Elisa Kit

Elisa kit negative control and positive control: If the negative and positive controls have correct results, they can provide a rough judgment of the results of the kit operation.

Blank control: For ELISA reagents, blank control is actually a control without adding samples or enzymes. The blank control mainly measures the absorbance of the system. Blank control is particularly important in single wavelength measurements

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4、 Precautions

Due to the sensitivity of substrate chromogens to light, they should be stored away from light.

2. The incubation time should strictly follow the instructions on the reagent kit.

3. Double well experiments should be conducted as much as possible to ensure the accuracy of the data and reflect the precision of the reagent kit.

When there are doubts about the results of the sample, other testing methods need to be used for verification.

5、 Proxy testing service

Customers can receive samples by express delivery, and customers in Shanghai can enjoy our on-site sampling service for sample testing.

The newly released reagent kit on our company's website can provide proxy testing services.

The reagent kits not yet listed on our company's website can be developed and tested on our behalf, and related information such as costs can be consulted by phone.