Soil Dehydrogenase (SDHA) Test Kit Diagnostic Kit

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Overview

Soil Dehydrogenase (SDHA) Test Kit $r $n Product Code: BLL-S8B906 $r $n Application: Qualitative/Quantitative/Enzyme Activity Detection $r $n Samples: Serum, plasma, saliva, urine, tissues, cell supernatants, lysate, etc. $r $n Suitable Species: Humans, mice, rats, plants, fish, shrimp, crabs, cows, sheep, dogs, cats, microorganisms, cells, soil and other animals and plants

Product Details

Product Name：Soil Dehydrogenase (SDHA) Test Kit
Brand: Baili Lai
Item number: BLL-S8B906
Specification: 50 tubes/24 samples
Detection method: spectrophotometric method
English name: SoilDehydrogenase (SDHA) TestBox
This product is only used for scientific research experiments and is not intended for clinical useBed.
Soil Dehydrogenase (SDHA) Test KitSample preparation:

Preparation of serum (plasma) samples

Serum and plasma are both liquid parts of blood that do not contain tangible components such as cells (including platelets). The main difference is that serum does not contain platelets, while plasma does. Their preparation methods are as follows:

1) Preparation of serum: The obtained blood cannot be anticoagulated. It should be placed in a centrifuge tube or a vessel that can be centrifuged, and allowed to stand or be placed in a 37 ℃ environment to promote coagulation. After the blood has coagulated, it should be equilibrated and centrifuged (usually 1000-2000g, centrifuged for 5-10 minutes). The supernatant obtained is the serum, which can be carefully aspirated (be careful not to aspirate cellular components) and used separately.

2) Preparation of plasma: First, add a certain proportion of anticoagulant (anticoagulant: blood=1:9) to a container containing blood. After adding a certain amount of blood, mix it upside down and centrifuge (centrifugation conditions are the same as above) to obtain the supernatant, which is the plasma. The first-time user should transfer the supernatant to another clean container and use a capillary pipette to gradually suck down the plasma while keeping it close to the liquid surface, avoiding being unable to suck up cellular components.
土壤脱氢酶(SDHA)测试盒

Preparation of tissue or cell homogenate samples

Collect tissue or cell samples, add homogenization medium, and perform homogenization. There are four homogenization methods to choose from.

1) Manual homogenization: Pour the sample (including homogenization medium) into a glass homogenization tube, hold the homogenization tube with your left hand and insert the lower end into a container containing ice water mixture. Insert the pestle vertically into the homogenization tube with your right hand and rotate it up and down dozens of times (6-8 minutes) to homogenize the tissue.

2) Mechanical homogenization: Weigh the tissue into an EP tube, add homogenization medium, and use a tissue homogenizer to grind it into tissue homogenate under ice water bath conditions at 60 Hz for 90 seconds. The homogenization time can be appropriately extended for skin, muscle tissue, and plant tissue.

3) Ultrasonic fragmentation: Use an ultrasonic generator to sonicate with an amplitude of 14 μ m for 30 seconds, and crush cells under ice water bath conditions; Alternatively, an ultrasonic disruptor can be used with a power of 200 W, 2 seconds per cycle, and a gap of 3 seconds for processing, with a total time of 5 minutes.

4) Repeated freeze-thaw cycle: suitable for cell samples, which involves resuspending cells in low osmolarity or double distilled water, and then repeating the cell suspension in a "freeze-thaw freeze" cycle for about 3 times.

It is worth noting that this method affects the activity of certain enzymes. Therefore, this method is not recommended for detecting enzyme activity in cell samples.
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