Super ECL Plus (ultra sensitive chemiluminescence detection kit)

Super ECL Plus (ultra sensitive chemiluminescence detection kit)

Product Code:S6009M

Product Specifications100 mL

Storage conditions:4Sealed at ℃ and stored away from light, can be kept at room temperature for a short period of time.The expiration date can be found on the outer packaging.

Product Introduction

Super ECL PlusImmunoblot substrate is enhanced chemiluminescence（ECL）The substrate of peroxidase can help users detect low expression or high-value proteins during immunoblotting analysis.Super ECL PlusThe substrate can be horseradish peroxidase（HRP）The immunoblotting experiment of the conjugate provides a bright signal and has high sensitivity for detecting low expression or high-value proteins. ThisECLThe substrate is compatible with various membranes, blocking solutions, and wide range antibody dilutions, meeting the immunoblotting application needs of users with excellent performance, versatility, and high cost-effectiveness.Super ECL Plus(Hypersensitivity) Characteristics:1. haveSensitivity: detecting nitrocellulose membrane orPVDFOn the membraneLow expression or high valueProtein bands;2. Long signal duration: under optimized conditions, the print incubated with substrateThe trace strip can continuously output6 To8 hDetectable optical signals;3. Price economy: The formula has been optimized and is suitable for detecting antibodies with extremely low concentrations.• 0.2-1 μg/mLPrimary antibody (or1 mg/mLThe dilution1:1,000-1:5,000）• 10-50 ng/mLSecondary antibody (or1 mg/mLThe dilution1:20,000-1:100,000）

Usage:

1. Execute routineSDS-PAGEElectrophoresis, membrane transfer, andWestern BlotStep by step,1.0-0.2 μg/mLIncubation of primary antibody at room temperature1 hOr4Overnight at ℃, after washing the membrane,10-50ng/mLSecondary antibody incubation30-60 min。2. Western BlotDuring the final washing of the film, freshly prepared luminescent working solution: divided intoDo not take equal volumes of solutionAAndBMix well.Note: It is recommended to use the working fluid immediately, and it can still be used after being left at room temperature for several hoursSensitivity has slightly decreased.3. Imager detection: remove with tweezersPVDFThe membrane is placed on the imaging detection boardProtein containing side up, drain dry cleaning solution but do not let the film dry. Apply luminescent working fluid（0.125 mLLuminescent working fluid/cm2 Add the film dropwisePVDFOn the membrane, make hairLight liquid coveragePVDFMembrane, room temperature incubation3-5 minRefer to the instrument instructionsCheck the book.4. Tablet testing: Use tweezers to remove itPVDFThe film is placed on top of the cling film and contains proteinFace up, drain dry cleaning solution but do not let the film dry. Apply luminescent working fluid（0.125 mLLuminescent working fluid/cm2Add the film dropwisePVDFOn the membrane, make the luminescent liquidCoveragePVDFMembrane, room temperature incubation3-5 minDiscard the luminescent working fluid and use it for preservationWrap the membrane and secure it in the clip, with the protein containing surface facing upwards. Pressing in the darkroom1 minDevelop immediately and adjust the pressing time based on the results. Or separately press the tablets0.5、1、3The5 minThen develop and observe the results together.

Notes:

1. Exposure of luminescent liquid to strong light for too long may reduce sensitivity, so be careful to avoid light during operation. Wearing gloves can avoid leaving fingerprints on the film.2. Long term exposure will deepen the background; Excessive protein can cause a loss of linear relationship between band strength and weakness; Insufficient exposure results in blurry or shallow stripes.3. If the strip is not good after exposure, the membrane can be washed with a washing buffer, the secondary antibody can be re incubated, and then reusedECLExposure.4. Use pre stained proteins visible to the naked eyeMarkerAnd fluorescence-The radiographic exposure label can accurately determine the position and size of the strip on the film.5. NaN3 Will inhibitHRPActive, recycled secondary antibodies should be avoided from useNaN3If necessary, do not exceed0.01%.