agarose

agarose

Product Code:A2015

Product Specifications100 g

Storage conditions:Store at room temperature, see outer packaging for expiration date.

Product IntroductionAgarosenamelyagarose, not includedDNaseTheRNaseAndProteaseIt is often used to prepare nucleic acid analysis gel, for exampleDNAOrRNAelectrophoreticagaroseGel, etc. Frequently usedTAEOrTBEAs an electrophoresis solution.agaroseThe concentration of gel andDNAThe resolution of electrophoresis and the ideal electrophoresis solution are shown in the table below. When the resolution requirement is not high, useTAE (Tris-Acetic acidEDTAbuffer solution)AndTBE (Tris-Boric acidEDTAbuffer solution)Both are acceptable; When the resolution requirement is relatively high, a lower concentration of glue is beneficial for improving the resolution of high molecular weight nucleic acids, and it is advisable to use it at this timeTAEA higher concentration of gel is beneficial for improving the resolution of small molecular weight nucleic acids, and it is advisable to use it at this timeTBE

Usage:

1. According to the electrophoresis requirements, prepare appropriate concentration electrophoresis and gel preparation buffer solutions. Note: The buffer used for electrophoresis and the buffer used for gel preparation must be the same.2. According to the gel making amount and gel concentration, add accurately weighedagarosePowder (the total liquid volume should not exceed that of a conical flask)50%Capacity).3. Heating and dissolving in a microwave ovenagaroseSet high heat to boiling and keep the glue boiling for about30 sWear heat-resistant gloves, remove the conical flask, carefully shake the flask, resuspend undissolved particles, and heat it again on high heat10-30 sOr untilagarosedissolution. Please wear heat-resistant gloves and carefully shake the triangular conical flask to ensure that the agarose gel solution is fully and evenly distributed. Note: It is necessary to ensureagaroseFully dissolved, at this pointagaroseClear adhesive solution,Otherwise, it will cause blurry electrophoresis images. Stop heating if the adhesive solution boils violently and foams during heating. The heating time in the microwave should not be too long.4. According to the experimental requirements, add a certain amount of nucleic acid electrophoresis dye and mix thoroughly. Note: It is recommended to use the product launched by our companyGelstainRedTM，Super GelBlueTMSafe and non-toxic nucleic acid gel electrophoresis dye.5. Translate into EnglishagarosePour the solution into the glue mold, and then insert a comb at the appropriate position. The thickness of gel is generally3-5 mmbetween.6. Let the glue solidify at room temperature (approximately)30 min-1 h）Then place it in the electrophoresis tank for electrophoresis. Note: When the gel is not used immediately, please wrap the gel with plastic wrap before4℃Save below, generally can be saved2~5Oh my god.

Quality Control:Gel strength（1%）：> 1200 g/cm2Electroosmosis（EEO）：< 0.15Sulfides:≤ 0.15%; gel temperature（1.5%Gel):35~37℃; Melt glue temperature（1.5%Gel):87~89℃； 水分：≤ 10%；

Nuclease cannot be detected