qiagen|RNase-Free DNase Set （50）

qiagen|RNase-Free DNase Set (50)

No RNase DNase set

Used for DNase digestion in RNA purification process

The RNeasy kit or QIAamp RNA Blood Mini kit, which does not require RNase, can efficiently perform on column digestion of DNA during RNA purification. Usually, RNA purified using RNeasy assay kit does not require DNase digestion, as silica gel membrane and rotating column techniques can effectively remove most of the DNA without DNase treatment. However, for certain RNA applications that are sensitive to very small amounts of DNA, it may be necessary to use DNase sets without RNase to remove DNA more efficiently. The RDD buffer included in the set has been optimized to perform on column DNase digestion at 20-30 ° C for 15 minutes. This buffer is also very suitable for effective DNase digestion in solution. For DNase treatment using RNeasy 96 kit, A 260 is 0.001 per milliliter per minute, at 25 ℃, pH 5.0, with highly polymerized DNA as substrate (Kunitz structure, M. [1950] crystalline deoxyribonuclease). I. Separation and general properties, determination of deoxyribonuclease activity by spectrophotometric method. II digestion of thymic nucleic acid (deoxyribonucleic acid): kinetics of this reaction J. Creatiphysiology. 33349 and 363).

qiagen|RNase-Free DNase Set (50)