12 oxo plant dienoic acid plant cell culture

12 oxo Phytodienoic Acid (OPDA)

Product label

OPDA 12 oxo phytodienoic acid; JA-Ile； c. 7-Epijasmonic acid; Plant immune hormone; Iasmonates (JAs) jasmonic acid; CAS NO.：85551-10-6；

Product Information

Product description

12 oxo phytodienoic acid (OPDA) is a direct precursor of 7-epi jasmonic acid and has biological activity. In addition to its function related to jasmonic acid activity, OPDA plays an independent role in mediating the body's resistance to pathogens and pests. As an endogenous signal transduction factor, OPDA can enhance the biosynthesis of alkaloids in E. californica cell cultures, increase the tendrils of B. dioica, and inhibit jasmonic acid-induced programmed cell death in A. flu mutant Arabidopsis.

Product Features

Preservation and transportation methods

保存：-80℃保存Valid for at least one year.

Transportation: Dry ice transportation.

Product Usage

1) This product is provided in the form of a mother liquor solution dissolved in ethanol. If other solvents need to be replaced, please place this product under a slow nitrogen flow to evaporate, and then add the required solvents such as DMSO and DMF. After evaporation and drying, OPDA can be dissolved in DMSO (~15mg/ml) or DMF (~25mg/ml); Due to the difficulty of directly dissolving OPDA in aqueous solutions, it can be first dissolved in strong alkaline buffer solutions (pH>8, ionic strength>0.1M). Take 400 μ l of strong alkaline buffer and add 1mg of OPDA. Shake vigorously and/or sonicate to dissolve it.

2) This product is only for scientific research purposes and should not be used for clinical diagnosis or treatment. It should not be used in food or medicine and is absolutely prohibited from being used on humans.

3) For your safety and health, please wear lab coats and disposable gloves when operating.

Related Products

background information(Jasmonic Acid Signaling Pathway): Reference (Ruan Jet al. Jasmonic Acid Signaling Pathway in Plants. Int J Mol Sci. 2019 May 20; 20(10). pii: E2479. PMID: 31137463）

1、 The concept and function of jasmonic acids (JAs)

Jasmonic acid (JA) is an endogenous plant regulator found in higher plants. Jasmonates (JAs), including jasmonic acid (JA), its methyl ester (MeJA), and isoleucine linker (JA Ile), are derivatives of a class of fatty acids. Zui was first identified as a hormone related to stress. Jas is also involved in regulating important growth and development processes. For example, Jas can induce stomatal opening, inhibit Rubisco biosynthesis, affect nitrogen and phosphorus absorption, and transport organic substances such as glucose. Especially, as a signaling molecule, JAs can effectively mediate environmental stress responses induced by a series of gene expressions. The signaling pathway mediated by JA and salicylic acid (SA) is mainly related to plant resistance, promoting plant response to external damage (mechanical, herbivorous, and insect damage) and pathogenic infections, thereby inducing the expression of resistance genes.

2、 Synthesis and metabolism of jasmonic acid compounds

The vast majority of research on the biosynthesis of jasmonic acid compounds (JAs) has been conducted in model plants such as Arabidopsis thaliana and tomato (L. esculentum). In Arabidopsis, there are three signaling pathways used to synthesize JAs, including the octadecane pathway starting from alpha linolenic acid (18:3) and the hexadecane pathway starting from hexaenoic acid (16:3). All three pathways require three reaction sites: chloroplasts, peroxides, and cytoplasm. Starting from unsaturated fatty acids, the synthesis of 12 oxo phytodienoic acid (OPDA) or dioxo-12-oxo phytodienoic acid (dn OPDA) occurs in chloroplasts and is then converted into JA by peroxides. In the cytoplasm, JA is metabolized into different structures through various chemical reactions, such as MeJA, JA Ile, CJ, and 12-OH-JA.

Fig 1. Scheme of the JAs biosynthesis pathway in Arabidopsis thaliana. The enzymes and the intermediates are indicated as follows: LOX for lipoxygenase, AOS for allene oxide synthase, AOC for allene oxide cyclase, OPR3 for OPDA reductase, JAR1 for jasmonate resistant 1, JMT for JA carboxyl methyltransferase; 18:3 for α-linolenic acid, 16:3 for hexadecatrienoic acid, OPDA for 12-oxo-phytodienoic acid, dnOPDA for dinor-12-oxo-phytodienoic acid, OPC8 for 8-(3-oxo-2-(pent-2-enyl)cyclopentyl) octanoic acid, OPC6 for 6-(3-oxo-2-(pent-2-enyl)cyclopentyl) hexanoic acid, OPC4 for 4-(3-oxo-2-(pent-2-enyl)cyclopentyl) butanoic acid, tnOPDA for tetranor-OPDA, 4,5-ddh-JA for 4,5-didehydrjasmonate, JA for jasmonic acid, JA-Ile for jasmonoyl-L-isoleucine, and MeJA for methyl jasmonate.

——Written/Edited by V. Shallan [Copyright belongs to MKBio Maokang]

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