AlgaeAI 700 plankton automatic classification and counting system

The AlgaeAI 700 plankton automatic classification and counting system developed by Xunshu consists of the AlgaeAI system and a fully automatic scanning microscopy system. With the help of a high-speed three-axis electric platform, high-definition microscopic images can be obtained. Using AlgaeAI technology based on super deep learning, species identification, classification and counting, biomass measurement, and cell density calculation of plankton in water can be automatically performed. Data reports can be generated and exported automatically, achieving electronic recording of plankton and ensuring the integrity of electronic data. The AlgaeAI 700 is also equipped with an intelligent identification expert module for plankton, providing an important tool for studying plankton diversity in aquatic ecosystems.
Artificial intelligence, within reach
AlgaeAI 700 is an advanced intelligent analysis and recognition computing system for planktonic organisms based on Xunshu's ultra deep machine learning. It is a data analysis technology that can be used by personnel conducting planktonic analysis without the need for deep expertise in planktonic biology.
·Simple, one click completion of identification, classification, counting, percentage, algae density calculation, dominant algae sorting, and report output
· Fast, single field analysis only takes 0.6 seconds
· Accurate and capable of identifying 145 common algae and 60 freshwater planktonic animals trained by neural networks, with a recognition accuracy of over 95%
· High robustness, suitable for complex microscopic images commonly seen in experiments: overlapping algal cells at high densities, local structural blurring caused by insufficient depth of field, background mixed with many impurities, cells only partially within the field of view.
Full transparency, visible reality
The analysis, recognition, and statistical process are fully displayed on the screen, and the operator can clearly observe the analysis process and processing results of each image.
· Click on 'AI Start', the main window images flash one by one, and the algae cells are framed one by one, with the name of the algae at the top of the box
· The green scrollbar on the upper right indicates that the sample is being tested
· On the right is real-time jumping updated data: phylum, algae name, algae quantity, percentage, algae density

Detection completed, click on the image queue in the bottom left corner to easily view the algae count on each image: individual names are incorrect? Individual algae not detected? Do individual algae need to be removed? Simple, click on the toolbox and fix it in 2-3 seconds.
Solving the difficulties of detecting complex field of view images
There are many challenges in the quantitative analysis process of phytoplankton, such as high suspended impurities in the collected quantitative water samples, high cell density after concentration, overlapping microalgae, existence of layered and divided fields of view of algal cells in the counting box, insufficient clarity of microscope optical imaging, inaccurate focusing
AlgaeAI 700 utilizes its super deep machine learning capabilities to quickly and reliably generate high-quality analysis and recognition results.

Not afraid of the crossing and overlapping of algae cells, it can be automatically untied directly

Samples with algae density exceeding 1010 cells/L and background clutter can still be recognized and counted

The light source and focus adjustment are not in place, and brittle rod algae and disk star algae are very shallow and light, but can still be recognized

Automatic identification of planktonic animals
The digital treasure trove of intelligent identification of algae
The grand algal image library covers freshwater and marine species in inland waters such as rivers, lakes, reservoirs and surrounding waters in China. The exquisite selected pictures and text introduction, combined with a rich retrieval framework, are helpers for the basic teaching of plankton and the popularization of algal knowledge by water environment monitoring organizations.

Taxonomic search, key editing and column introduction of common algae, with clear understanding of morphology, structure, reproduction, and ecology.

Morphological retrieval, based on morphological similarity and gradient, combines and classifies into graphic language, and combines the structural characteristics of cells or populations, such as flagella, pigment bodies, patterns, gelatinous covers, etc., to achieve accurate and fast morphological retrieval.
High quality microscopic scanning imaging
Using the Olympus research grade biological microscope BX43 as the optical imaging carrier, equipped with a smooth and quiet XYZ electric stage, achieving one click precise operation: automatic focusing, automatic scanning, and excellent image quality.

Main functions and technical indicators
1. Analysis standards
Comply with the technical regulations for monitoring phytoplankton in inland waters SL733-2016, technical requirements for aquatic ecological monitoring - freshwater phytoplankton, HJ1216-2021 water quality phytoplankton determination 0.1mL counting box microscope counting method, HJ1215-2021 water quality phytoplankton determination filter membrane microscope counting method, water and wastewater monitoring and analysis methods (fourth edition), and the corresponding algae analysis requirements of GB17378-2007 marine monitoring specifications.
2. Fully automatic scanning microscopy system
Olympus BX43 microscope: Objective lens specifications 4, 10, 20, 40 times and a half apochromatic objective lens
High precision electronic control XYZ automatic scanning platform: realizing micrometer level motion and automatic control in X/Y/Z axis directions
Stepper motor XY platform: load 4 pieces at once, minimum step size ≤ 0.1um, bidirectional repeat positioning accuracy ≤± 1um, maximum speed: 20mm/s
According to the adjusted density of phytoplankton in the sample, imaging can be performed using methods such as whole sheet scanning, grid scanning, and random field scanning
Electric Z-axis: closed-loop resolution of 0.156um; repeat positioning accuracy: ≤± 0.4um
High sensitivity global shutter camera, multi depth continuous automatic scanning focus, adjustable shooting layer spacing, image resolution<0.20um/pixel
3. AlgaeAI 700 is a super deep learning based fast counting plankton AI system. The fast counting AlgaeAI 700 plankton AI automatic classification and counting system is developed by an expert team based on in-depth research on plankton characteristics and machine learning theory. It innovatively establishes a robust artificial intelligence analysis system to achieve automatic classification and counting, size measurement, and biomass determination of algae and plankton.
It can automatically identify algae ranging from 3 to 1000 μ m, including more than 145 common algae such as Chlorophyta, Cyanobacteria, Diatom, Cryptophyta, Cyanobacteria, Cyanobacteria, Chrysophyta, and Naked Algae. The algae density detection range is 9.2 × 102-1011 cells/L. Single field automatic recognition and analysis time is ≤ 0.6 seconds, achieving accurate algae recognition, classification and counting, and synchronously completing dominant algae sorting and biomass calculation.
The dominant species recognition rate of the local classification recognition library is ≥ 95%, and the repeatability error of automatic analysis is ≤ 5%
One click operation, full process dynamic visualization: main window images line up and move rapidly, algae cells are instantly recognized, and names are labeled in situ; Real time fluctuation updates of detection data (category, name, quantity, percentage, algal density, etc.); The green scrollbar displays the progress of image collection detection. Transparent operation throughout the process, facilitating quality monitoring. Mouse interaction allows for the addition, deletion, and modification of recognized species information, and real-time updates of sample analysis results.
Sort the statistical data by dominant species, displaying the category, Chinese name, Latin name, number, proportion, and density of phytoplankton. Calculate the average single-cell length, single-cell width, single-cell height, single-cell diameter, single-cell area, and single-cell volume of the species, and automatically calculate biomass, total biomass, Shannon index, species evenness index, biodiversity index, abundance, and dominance.
Electronic records, data traceability, and reporting: Automatically save data and generate statistical reports with just one click. The completed analysis results are saved, and the algae names are marked in situ on the collected images. At any time, the statistical accuracy of each image can be reviewed again by opening the file.
High robustness: It has anti-interference ability. For field of view images containing a large amount of impurities, even if algae cells are in the impurities, this system can accurately identify them based on strong reasoning ability.
Separation and recognition of overlapping/adherent algae: AlgaeAI 700, based on intelligent adhesion separation technology, can accurately capture individual algae cells from a pile of adherent cells that are highly overlapping together.
Intelligent recognition of incomplete/local algae: AlgaeAI 700, based on intelligent morphological reasoning technology, can accurately identify the type of algae at the edge of the field of view based on local information, thus achieving leak free detection.
Fuzzy cell prediction and recognition: AlgaeAI 700 uses fuzzy prediction technology to accurately analyze and recognize algae cells that appear faint and unclear in the field of view due to insufficient depth of focus.
Plankton analysis module: relying on powerful AI image recognition technology, a high-precision neural network mathematical model is constructed to accurately identify 65 species in water, automatically measure indicators such as plankton body length and width, calculate density and biomass, issue detection reports, and achieve paperless data recording. In addition, the system provides an information database that includes text, hand drawn images, and microscopic photographs. It is equipped with classification information and keyword search functions, which can display zooplankton with both text and images.
4. Classic image segmentation counting module
Dynamic automatic counting: Seven segmentation algorithms used for pre checking multi view counting and adjusting phytoplankton density to 107-108 per liter
Spherical like colony cell automatic counting: automatically identifying and counting daughter cells in the colony, especially suitable for counting and analyzing Microcystis aeruginosa
Estimation of filamentous cells: used to estimate the number of daughter cells in a single filamentous or chain like body
5. Qualitative analysis and intelligent identification module for plankton
Plankton Expert Database: Composed of exquisite color micrographs, hand drawn images, bilingual displays in Chinese and Latin, it forms freshwater and marine plankton databases that can be searched at four levels: phylum, order, genus, and species. Among them, there are 15 phyla and 1700 genera of algae; There are 26 major categories and 2000 genera of planktonic animals. Freshwater algae covering the plain lake area in eastern China, the Yunnan-Guizhou Plateau lake area, Northeast lake area, Qinghai Tibet Plateau lake area, Mongolian Xinjiang Plateau lake area and seven major water systems, as well as marine algae around the East China Sea, Yellow Sea, Bohai Sea and South China Sea
Typical combination association "morphological retrieval: using graphic language, combination association, and combining the structural characteristics of cells or populations to achieve accurate and fast morphological retrieval. Equipped with features such as multiple selection, freshwater and ocean storage, and easy browsing, beginners can quickly master them.
Multi dimensional progressive similarity algae search and identification: an automatic and intelligent algae cell graphic recognition tool that can detect unknown algae cell contours, extract feature information, match big data, accurately identify possible algae with similar morphology, and display the most similar common algae synchronously under the "priority selection" option in 3-5 seconds.
Identification of easily confused algae: Designed for inexperienced experimenters, screen multiple algae that are easily confused due to their similar morphology, conduct quick comparisons on the same interface, and quickly grasp the distinguishing points through typical feature puzzles and summary text.
6. Configuration List
One set of AlgaeAI 700 algae and zooplankton intelligent analysis system for Xunshu
1 set of fully automatic digital microscope image scanning system: Olympus BX43 microscope, UIS2 infinite optical system, 4X, 10X, 40X flat field semi apochromatic objective lens, 20X full apochromatic objective lens (numerical aperture 0.75), 10 times refractive power adjustable eyepiece, three eye observation tube, 5-hole object mirror converter, 4 flux high-precision electronic XYZ automatic scanning platforms and control boxes, high-sensitivity global scanning camera
1 data analysis workstation: 12th generation intelligent Intel Core i9-12900 16 core, 32GB DDR4 memory, 4G discrete graphics card, 512GB solid-state drive, 4T hard drive, 27 inch display, Windows 10 Professional operating system
7. Service
New machine on-site installation, debugging, and training
Build a free local database algorithm for users once
Long term provision of remote assistance guidance services and assistance in identifying complex samples