HD5000 Multispectral Super Resolution Colony Imaging System Nuclear Radiation Detector

HD5000 Multispectral Super Resolution Colony Imaging SystemMain functions and technical indicators

• lighting system

• Fully enclosed steel aluminum alloy chassis (32 × 34 × 46cm): precise and sturdy, ensuring a light tight seal

• Flat dish sample compartment: Pull down aluminum alloy partition window to eliminate environmental stray light interference, block UV leakage, and prevent dust from entering

• Misty diffuse reflection lighting

1. 96 LED arrays and nano reflective materials form an embedded fog light system, with 360 ° continuous diffuse reflection, highlighting the color and texture of bacterial colonies, and eliminating the light spots and halos formed by the refraction of glass culture dishes.

2. Color temperature variation range: 3100K-5800K Illumination range 50-7000 Lux

3. LED lifespan ≥ 20000 hours

• Floating dark field illumination

White LED light source, illumination range 100-5500 Lux, color rendering index 74%

• Color (12 colors) transparent backlight illumination

1. Adjustable LED light guide array, forming uniform and bright 12 color transmitted light

2. Illumination uniformity greater than 90%, ensuring uniform illumination at the edges and center of the culture dish

• Multi spectral Rheinberg lighting

1. 12 channel visible excitation light, circular screen reverse transmission, and 156 combination lighting modes can be formed with oblique backlight

2. Multispectral mode can reduce the influence of uneven culture medium and color change, and minimize the interference of agar impurities

3. The combination of non-destructive light staining technology and anti-interference precision statistical technology enhances the identification of subtle color differences between colonies, significantly improving the accuracy of colony recognition and screening

• UV reflective light source: 254nm used for cavity disinfection and UV mutagenesis; 366nm can be used for fluorescence excitation

• light source controller

1. Invisible suction control panel, 6-way lighting selection switch, 4-channel stepless brightness adjustment, dual channel color temperature adjustment

2. Free switching of lighting combinations

• digital imaging

• 1.1-inch large target high-definition industrial fixed focus lens, with a resolution of 150 lp/mm maintained at both the center and edges of the lens

• Ultra large array CMOS camera: SONY 4/3-inch color CMOS sensor,

Resolution: 21 million pixels

Single pixel size: 4.54X4.54um

• Colony analysis module

1. Basic colony counting function

• Type of petri dish: pouring, coating, membrane filtration, spiral petri dish, 3M paper sheet

• Whole dish colony count: Count the total number of colonies and display them classified by 25 sizes

• Region selection statistics: You can choose circular, rectangular, or arbitrarily delineated regions for statistics

• Multi domain parallel statistics: synchronous statistics of multiple regions at once; Multi regional "hollow out" statistics

• Diameter classification statistics: Set the diameter range and count specific sizes of colonies

• Mouse click statistics: Quickly mark and add colonies, suitable for counting colonies at the edge of culture dishes

• Colony adhesion segmentation: Automatically segment colonies that adhere to each other, and users can choose to segment or not segment chain colonies

1. Rapid colony counting

• Roller parameter adjustment statistics (4 types): homogeneous petri dish, uneven background, small bacterial colonies, colored background

• One click response statistics (3 types): monochrome statistics, mold statistics, and trans statistics

1. Advanced colony statistics

• Dynamic adjustment statistics: The statistical results can be dynamically adjusted and corrected to quickly obtain statistical effects.

• Deviation estimation statistics: suitable for situations with multiple and complex colony colors.

• Level set multi model algorithm: search operation, obtain image segmentation effect, adapt to background changes in the culture medium

• Specific colony statistics: Identify specific colonies based on their color, size, and contour characteristics

• Trans statistics: suitable for extremely complex colony types and uniform culture medium background

• Statistics of high adhesion bacteria: segmentation calculation suitable for multiple adhesion bacteria

• Removal of miscellaneous bacteria and impurities: Automatic removal of miscellaneous bacteria and impurities based on differences in morphology, size, and color

• Spiral colony statistics: the spiral plate is automatically counted according to FDA standards, and supports exponential mode, slow exponential mode, uniform mode, proportional mode, lawn mode, etc. Compatible with SBI in the United States and IUL in Spain.

1. Grid filter membrane and 3M test piece

• Black solid line grid, one click statistics

• 3M total bacterial count test piece, 3M Staphylococcus aureus test piece: one click statistics

• 3M coliform test strip, 3M Escherichia coli/coliform rapid test strip: one click statistics+manual selection

1. Typical bacterial screening

• Monochromatic classification statistics: Select specific bacterial colonies based on color accuracy, diffusion, colony size, and contour features

• Multi color automatic clustering: automatically distinguish 24 different colored colonies based on color clustering accuracy

• Multi color screening: screening 1-8 color colonies at once

• Transparent circle characteristic analysis: suitable for antibacterial circle, hydrolysis circle, discoloration circle, calcium dissolution circle, hemolysis circle, oil discharge circle, phosphorus dissolution circle analysis

• Dual color circle automatic filtering

1. Description of colony characteristics

• Bacteria and yeast: features such as color, size, shape, surface morphology, edge, gloss, transparency, etc., intelligently described and sorted

• Mold and actinomycetes: features such as front color, back color, size, surface morphology, edges, texture, etc., intelligently described and sorted

1. Microbial limit analysis tool

• Applicability check of culture medium

• Control bacterial examination - colony morphology

1. HD5000 Multispectral Super Resolution Colony Imaging Systemspecialized analysis

• Mold proof testing: quantitative analysis of mold proof level

• Multi region serial statistics: suitable for complex bacterial colonies with uneven background in the culture medium

• Multi region parallel statistics: suitable for porous plate, OPKA, SBA analysis

1. Advanced Tools

• Grid cleaning: eliminate background interference in the filter membrane grid

• Manual counting correction: adding or deleting colonies

• Exclude contaminated areas: Use the mouse to outline any contaminated area and automatically remove the bacterial count from the contaminated area

• Background text removal: automatically eliminates marker interference

• Artificial adhesion segmentation: manually segmenting multiple adhesive colonies

• Automatic parameter conversion: Input the diameter of the culture dish and sample dilution to achieve automatic conversion

• Text and graphic annotation: various drawing tools and embedded Chinese and English text

1. Calibration and Measurement

• Instrument calibration: The instrument comes with built-in calibration and manual correction calibration

• One click rapid measurement: One click determination of large bacterial colonies, suitable for single colony analysis of fungi and actinomycetes

• Automatic measurement of the entire dish: analysis of the equivalent diameter, area, length, circumference, and roundness of the entire dish colony

• Multi directional ruler measurement, manual precise measurement: length, angle, radian, area, arc, arbitrary curve

1. image processing

• Image adjustment: grayscale image and negative phase diagram conversion; Brightness, contrast, and saturation adjustment; RGB adjustment

• Image enhancement: sharpening, adaptive enhancement

• Image filtering: median filtering, high pass filtering, Gaussian filtering, low-pass filtering, queue filtering, high pass Gaussian filtering

• Edge detection: Sobel operator, Robert operator, Laplace operator, vertical detection, horizontal detection

• Morphological operations: erosion, dilation, opening operation, closing operation

• Data Security and Management

1. The four tiered system architecture of "system, data, operation, and review" is divided into functions and permissions to ensure the security, integrity, and authenticity of data information

• System administrator: responsible for creating and managing accounts and login passwords for all operators and auditors. Ensure account and data isolation between operators and between operators and auditors.

• Data administrator: responsible for archive management of all test data and database management of computers. Seal all approved test reports or backup and export original images and test data to ensure data integrity and security.

• Operator: Responsible for testing, self checking, correcting, generating electronic reports, submitting for review, and printing reports on approved documents for culturing dish colonies.

• Reviewer: Responsible for reviewing the test reports submitted by operators. Verify the data input and processing process, but have no authority to modify it; Review and return the suspicious report, and request the operator to retest it; The "approved" report will be archived, and neither the auditor nor the operator has the right to delete it again to ensure the originality and authenticity of the data.

1. Data storage and export

• Mainly based on electronic data, record: sample source, number, dilution, petri dish image, recognition effect, count value, statistical tools used, parameter settings, correction status, to ensure complete record information.

• Meet quality audit requirements, and the stored electronic data can be printed and output in PDF or Excel format

1. Watermark signature technology, tamper proof technology, and intelligent reconstruction technology for testing processes to achieve effective audit tracking

• Anti tampering technology

1. Adopting multi-user login management, the names of all operators and auditors are automatically recorded in the operation process and test report by the system; All operation dates and review dates are automatically generated by the computer to avoid errors or forgeries.

2. The entire operation process, including colony images, dish size, sample dilution, statistical tools, parameters used, total bacterial count obtained from testing, self checked and corrected total bacterial count, etc., is automatically recorded in the database by the computer and cannot be modified by the operator, providing all real data for subsequent audits.

• Watermark signature technology

The test report that has been "approved" will automatically generate electronic signatures for the operator and auditor's accounts, and an anti-counterfeit "approved" watermark will be printed on the report.

• Intelligent refactoring technology for testing process

1. The 'reviewer' opens the 'waiting for review' test record, and the computer automatically restores the entire process and testing environment of the operator, including: images of the culture dish measured at that time, test results, dish size, sample dilution, statistical tools and parameters used, total bacterial count obtained from the test, correction status

2. By reproducing the testing environment and testing process, the reviewer can trace all the operations of the operator, verify the accuracy of the test results, and achieve the purpose of audit tracking.

• Antibacterial zone analysis module

1. Multi mode measurement technology for Szone inhibition zone

• Automatic detection: precise edge detection based on the contour of the antibacterial zone, suitable for clear edges and circular antibacterial zones

• Quasi circular approximation: Based on the contour of the antibacterial zone, circular fitting approximation is suitable for edge rupture and non-standard circular antibacterial zones

• Manual detection: Click on three points on the edge of the antibacterial zone with the mouse to form a circle, suitable for antibacterial zones with blurred edges

1. Antibiotic potency determination

• One dose method potency testing: suitable for the United States Pharmacopeia

• Two dose method, three dose method, and combined calculation: suitable for the 2020 edition of the Chinese Pharmacopoeia

• Repetitive self check: relative error ≤ 0.01%, repeated measurement accuracy ≤ 0.002mm

• Uniformity self check: relative error ≤ 0.05%

• Measurement difference between stations ≤ 0.2%

1. Sulbactam sensitive β - lactase test

• Pure water verification: Generate inhibition zones based on (A), (B), and (D), D-C≧3， B-A≦3 ， The judgment system is established

• Automatically detect the (A), (B), (C), and (D) inhibition zones of three parallel samples and import the data

• Automatically calculate the average value of parallel experiments and intelligently distinguish the positive and negative results.

• Invalid report automatic warning

• Instrument specifications and configuration

• 1 mainframe of multispectral super-resolution colony imaging system

• Bacterial colony analysis software, automatic inhibition zone measurement software, antibiotic potency determination software, sulbactam sensitive β - lactase testing software

• Integrated computer: Dual core quad thread CPU/4G memory/1T hard drive/23 'high-definition screen, Windows 10 system