HEP-53.4 mouse liver cancer cells

Cell name:HEP-53.4 mouse liver cancer cells

Cell alias:HEP-53.4 ; 53.4 ; Mouse liver cancer cells

Cell source:Germany

Cell identification:STR identification has been passed

Cell morphology:Epithelioid cells, adherent growth

Culture medium:DMEM (containing NaHCO3 1.5g/L) (BasMed-AW-013)+FBS 10%+P/S1%

Cultivation conditions:Gas phase air, 95%; Carbon dioxide, 5%. Temperature: 37 degrees Celsius, humidity in the incubator is 70% -80%

Freezing conditions:Serum free cryopreservation solution, stored in liquid nitrogen

Cellular background:Primary hepatocellular carcinoma originating from C57BL/6J mice, these mouse liver cells faithfully represent hepatocellular carcinoma and provide valuable models for studying this type of liver cancer, synonymous with HEP-53.4 and 53.4, which are easy to identify and cross reference. Hepatocellular carcinoma is a major health issue, and these cells can be accurately studied for their molecular pathways, cell interactions, and treatment strategies. These cells originate from Mus musculus (mice) and provide a relevant model system for understanding and developing treatment methods for hepatocellular carcinoma.

Cell usage:For scientific research purposes only

Cell delivery time：In stock, about a week

Cellular issues

Shipping at room temperature

upon receiptDisinfect the T25 bottle and place it in the incubator for 2-3 hours. Observe the density and condition, take 2-3 photos, and provide feedback to the sales team. Once the density meets the standard, it can be passaged. The initial passage ratio is 1:2. It is recommended to freeze one whole bottle into a 1ml cryovial and continue passaging the other bottle. Repeat the freezing process for 2-3 bottles before amplification for experimentation to prevent seed breakage in case of unexpected situations.

Dry ice shipment

Conventional Cell Shipping CryovialTwo, one recovered and the other kept as a backup. If the first one fails to recover, strictly follow the manufacturer's requirements to recover the second one. If there is no successful recovery, please keep a photo of the recovery and notify us immediately.

Precautions

1. Conventional digestion involves collecting cells and centrifuging them.

2.After centrifugation, remove the supernatant from the centrifuge tube, add 1ml of resuspended cells and mix well. It is recommended to gently shake or blow the cells, place them in the incubator for digestion, and digest for about 1 minute.

3. After digestion, gently blow the cell suspension with a pipette to disperse the cell clusters. Quickly add 3-5ml of serum containing culture medium and mix well to terminate digestion,.

5.Observe under a microscope to see if the cells are evenly dispersed as single cells. If there are a small number of clustered small cell clusters, they do not need to be digested again. Let them adhere to the wall and wait for the cells to grow stably before dissipating.

4. Add about 5ml of the corresponding culture medium to the cells and mix well. Transfer to the culture bottle/dish in proportion.

adherent cells

1. Discard the culture supernatant and use one that does not contain calcium or magnesium ionsWash cells with PBS 1-2 times.

2. join0.25% (w/v) EDTA is placed in culture bottles (T25 bottle 1-2mL, T75 bottle 2-3mL) and digested in a 37 ℃ incubator for 1-2 minutes (difficult to digest cells can be appropriately prolonged for digestion time). Then, the digestion of cells is observed under a microscope. If most of the cells become round and fall off, they are quickly taken back to the operating table and lightly tapped a few times before adding 3-4ml of culture medium containing 10% FBS to terminate digestion.

Special attention

The cell grows well in DMEM (containing 1.5g/L NaHCO3) medium, and most DMEM containsHigh concentration NaHCO3 (3.7g/L), if DMEM (3.7g/L NaHCO3) medium is used to culture fineCellular time requires an increase in CO2 concentration (7% -10%)