LWnt-3A cell specific culture medium

LWnt-3A cell specific culture mediumProduct Introduction

After careful optimization by the team and long-term testing, this product can maintain the growth status of L Wnt-3A cells.

This product already includesThe various components required for the growth of L Wnt-3A cells can be directly used for the culture of L Wnt-3A cells without the need for any additional ingredients.

Main components of the product

DMEM basic culture medium 445ml

Defined FBS 50 ml

P/S Penicillium su Streptomycin su 5 ml

G-418 0.4mg/ml

Transportation and storage

Transportation: Low temperature transportation in an insulated box containing biological ice packs

保存方法： Store at 2 ℃~8 ℃, away from light, for 1 month; -Store at 20 ℃, away from light, for 3 months.

quality control

For scientific research purposes only.

Some components in the culture system are harmful to human health. Please do not use exposed skin to come into contact with the liquid in the culture system or containers containing residual liquid in the culture system; The concentration and harmfulness of these harmful substances are relatively low. If there is contact, rinse immediately with tap water.

The following experimental protocol introduces the general process of cell culture cryopreservation. A detailed experimental plan must refer to the product manual for specific cells.

1. Prepare frozen culture medium and store it at 2 ° C to 8 ° C until use. Please note that the type of cryopreservation medium used depends on the cell line used.

When freezing adherent cells, gently detach the cells from the tissue culture container using the method used during passage. Resuspend the cells in the required culture medium.

3. Use a hemocytometer or cell counter according to the trypan blue exclusion method or use Countess ® The automatic cell counter measures the total cell count and percentage of live cells. Calculate the required amount of frozen culture medium based on the required density of live cells.

4. Centrifuge the cell suspension at a centrifugal force of approximately 100-200 × g for 5 to 10 minutes. Carefully discard the supernatant under sterile conditions without stirring the cell sediment.

Note: The centrifugation speed and time depend on the type of cell.

5. Resuspend the cell pellet in pre cooled frozen culture medium and adjust it to the appropriate live cell density for the cell.

6. Divide the cell suspension into several cryovials. When packaging, cells should be gently mixed from time to time to maintain a uniform cell suspension state.

7. Use a freezing device with controllable cooling rate to freeze cells, reducing the temperature by approximately 1 ° C per minute. Alternatively, place the cryotube containing the cells into a cryobox and place the cryobox overnight at -80 ° C.

The research object is living cells

During the experimental process, cell viability can be maintained as required, and the condition of some live cells can be monitored, detected, and even quantitatively evaluated for a long time, including their morphology, structure, and life activities.

2. Research conditions can be artificially controlled

pH、 The physical and chemical conditions such as temperature, oxygen, carbon dioxide, tension, etc. can be artificially controlled according to actual conditions. At the same time, chemical, physical, biological and other factors can be applied as conditions for experimental observation, and these factors can also be strictly controlled.

3. The research sample can achieve relatively uniformity

After a certain number of generations of cell culture, the obtained cell lines can achieve uniformity and belong to the same type of cells. If necessary, methods such as cloning can be used to purify the cells.

4. The research content is easy to observe, detect, and record

Various research techniques are used, such as inverted biomicroscopy, fluorescence microscopy, electron microscopy, flow cytometry, confocal laser microscopy, immunohistochemistry, in situ hybridization, isotope labeling, and other instruments and equipment. The recording methods can include photography, time-lapse movies, television, and other means.

5. The scope of the research is relatively broad

The applied disciplinary fields are relatively broad, such as cytology, immunology, oncology, biochemistry, genetics, molecular biology, etc;

The scope of applicability is wide, from lower animals to higher animals, and targeted research can be conducted on different age stages and tissues of an animal.

6. The cost of research is relatively economical

Can provide a large number of experimental subjects with similar biological characteristics, same period, good repeatability.