M320 Colony Counting/Plankton Analysis Combination Instrument Algae Counting Instrument

M320 Colony Counting/Plankton Analysis Combined DeviceMain functions and technical indicators

1、 Colony digital imaging

1. Light source

Visible light: High brightness tri color LED structured light

254nm UV: used for cavity disinfection and UV mutagenesis

2. Light path and lighting control

Fully enclosed dark box: eliminating environmental stray light interference

Upper light source: scene style 360 ° flexible shadowless lighting

Lower light source: Crystal Sharp floating dark field illumination

Up light, down light, dual light, UV, freely switchable, adjustable light intensity

3. Optoelectronic conversion

Standard definition industrial fixed focus lens: 8mm, 3.0 mega pixel, 1/2 ", Distortion<1%, F1.4-F32

Professional CMOS camera: 1/2.3 "color CMOS sensor, 8.5Mega Pixels, C-Mount

2、 Colony counting module

1. Basic colony counting function

Type of petri dish: pouring, coating, membrane filtration, 3M paper sheet

One click intelligent counting (6 modes): flat sensing mode, three-dimensional sensing mode, small colony priority, large colony priority, same color bacteria priority, culture medium removal mode

Whole dish colony count: Count the total number of colonies and display them classified by 25 sizes

Region selection statistics: You can choose semicircles, rectangles, sectors, or any delimited region for statistics

Diameter classification statistics: Set the diameter range and count colonies of specific sizes

Mouse click statistics: Quickly mark and add colonies, suitable for counting colonies at the edge of culture dishes

Colony adhesion segmentation: Automatically segment colonies that adhere to each other, and users can choose to segment or not segment chain colonies

2. Advanced colony counting function

Dynamic adjustment statistics: The statistical results can be dynamically adjusted and corrected to quickly obtain statistical effects.

Deviation estimation statistics: suitable for situations with multiple and complex colony colors.

Level set multi model algorithm: search operation, obtain image segmentation effect, adapt to background changes in the culture medium

Specific colony statistics: Identify specific colonies based on their color, size, and contour characteristics

Trans statistics: suitable for extremely complex colony types and uniform culture medium background

Removal of miscellaneous bacteria and impurities: Automatic removal of miscellaneous bacteria and impurities based on differences in morphology, size, and color

3. Grid filter membrane and 3M test piece

Black solid line grid with one click statistics

3M total bacterial count test piece, 3M Staphylococcus aureus test piece: one click statistics

3M Escherichia coli test strip, 3M Escherichia coli/Escherichia coli rapid test strip: one click statistics+manual selection

4. Advanced tools

Grid Clearing: Eliminating background interference in the filter membrane grid

Manual counting correction: adding or deleting colonies

Exclude contaminated areas: Use the mouse to outline any contaminated area and automatically remove the bacterial count from the contaminated area

Background text elimination: automatically eliminates marker interference

Artificial adhesion segmentation: manually segmenting multiple adhesive colonies

Parameter automatic conversion: Input the diameter of the culture dish and sample dilution to achieve automatic conversion

Text and graphic annotations

5. Calibration and measurement

Instrument calibration: The instrument comes with built-in calibration and manual correction calibration

One click rapid measurement: One click determination of large bacterial colonies, suitable for single colony analysis of fungi and actinomycetes

Automatic measurement of the entire dish: analysis of the equivalent diameter, area, length, circumference, and roundness of the entire dish colony

Manual precise measurement: length, angle, radian, area, arc, any curve

6. Database module

Data storage and intelligent querying

Data export: Export statistical results in Excel or PDF files

Data Security: Operator Access Permissions, Data Modification Permissions Settings

3、 Microscopic imaging of plankton

Research grade color digital camera: SONY 12 megapixel CMOS sensor; 1/1.7 inches; G light sensitivity 280mv with 1/30s; FPS/Resolution: 7.1@4000x3000 ； 30@2048x1080 Exposure time: 0.244ms~2000ms; USB3.0

Microscopic imaging: real-time dynamic observation, fast capture, batch image saving

3D depth fusion: Quickly fuse different focal planes to solve the problem of local blurring caused by the distribution of algae cells in different liquid layers, and obtain panoramic deep and high-definition images of algae cells

Super View Splicing: Multi View Horizontal and Vertical Automatic Splicing

4、 Plankton database

1. Algae Expert Database

Composed of exquisite color micrographs, hand drawn images, and textual introductions, it forms freshwater and marine algae reservoirs; Covering freshwater algae in China's seven major water systems, 28 key lakes and reservoirs, as well as marine algae around the East China Sea, Yellow Sea, Bohai Sea, and South China Sea.

2. Zooplankton database

Composed of 24 major categories including protozoan flagellates, protozoan meat footed insects, protozoan ciliates, rotifers, branchiopods, and copepods, displayed in both Chinese and Latin, accompanied by written introductions, hand drawn illustrations, and numerous microscopic photographs of planktonic animals.

5、 Plankton identification

1. Algae intelligent identification - "Quick Search" module

Progressive Similar Algae Search: An automatic and intelligent algae cell graphic recognition tool that can detect unknown algae cell contours, extract feature information, match big data, and accurately identify possible algae with similar morphology in 3-5 seconds. The "Priority Selection" option synchronously displays the most similar common algae.

Identification of easily confused algae: Designed for inexperienced experimenters, multiple algae that are easily confused due to their similar morphology are screened and quickly compared on the same interface. Through typical feature puzzles and summary text, the distinguishing points are quickly grasped.

Typical combination association "morphological retrieval: using graphic language, combination association, and combining the structural characteristics of cells or populations to achieve accurate and fast morphological retrieval. Equipped with features such as multiple selection, freshwater and ocean storage, and easy browsing, beginners can quickly master them.

Four level taxonomic search: composed of exquisite color micrographs, hand drawn images, and textual introductions to form freshwater and marine algae pools; Freshwater algae covering seven major water systems and 28 key lakes and reservoirs in China, as well as marine algae around the East China Sea, Yellow Sea, Bohai Sea, and South China Sea; Expand the search by four levels: door, order, genus, and species.

Column editing: The names, classification status, morphology, structure, and reproductive ecology of phytoplankton are clear at a glance.

General search: Keyword search (based on the feature words in the algal cell text description); Common algae queries (algal blooms, red tides, toxic algae); Name query (Chinese name, Latin name)

2. Auxiliary identification of planktonic animals

Search by Chinese or Latin name

Select a class or genus to display all planktonic animals under that category

6、 Plankton Counting and Analysis

1. Process based counting of planktonic organisms

Classification and statistics of plankton: Various organisms are marked with color circles of different colors and sizes, clicked by category, and automatically accumulated and counted

Algae Total Count Statistics: Automatically accumulate the total number of various organisms in the sample, automatically sort dominant species, sort by phylum, and analyze the percentage of dominant community composition

Automatic calculation: algae density, biomass, Shannon Wiener index, species evenness index, dominance, abundance

Gel population analysis: automatic recognition and counting of daughter cells in the population, especially suitable for counting analysis of Microcystis aeruginosa

Chain like body analysis: used to estimate the number of daughter cells in a single filament or chain like body

2. Automatic counting of single-cell microalgae

Dynamic automatic counting: seven segmentation algorithms adapted to changes in single-cell microalgae and imaging backgrounds

3. Measurement and biomass analysis

Microscopic measurement: You can choose between transparent and opaque rulers, or directly click the mouse to draw a line to measure biological cells

Biomass analysis: automatically calculate biomass based on mathematical models of plankton morphology

7、 Experimental data security

Multi account hierarchical management, administrators and experimenters have different permissions to avoid tampering with experimental data

Electronic record keeping method is used to store the database, ensuring the integrity of the data

Database: Automatically save each batch of micrographs, statistical identification, and statistical data

Annotation: Any text or size annotation can be made on the captured images of planktonic cells

Report editing and printing: providing report writing templates, text input, and print preview

Data export: Export statistical data and images to EXCEL or PDF files

8、 Instrument specifications and configuration

? NewM320 Colony Counting/Plankton Analysis Combined Device1 host

Colony analysis software, algae analysis software, planktonic animal analysis software

Lenovo all-in-one computer (nationwide warranty): dual core CPU/4G memory/1T hard drive/21.5 'color display/DVD burning/wireless network card, Windows 7 or Windows 10

Microscopic Camera Research Grade Color CMOS

User optional: microscope and camera adapter