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Guangzhou Institute of Microbiology Group Co., Ltd
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Guangzhou Institute of Microbiology Group Co., Ltd

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    kf@gimgc.com

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    13822200442

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    1st Floor, Building J5, No.1 Lingtai Mountain Road, Science City, Huangpu District, Guangzhou City, Guangdong Province (Marketing Department)

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Medical wastewater quality testing

NegotiableUpdate on 03/14
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Overview
The water quality testing of medical wastewater needs to rely on scientific principles and standardized processes, combined with precision instruments and strict quality control, in order to accurately reflect the water quality status and provide reliable data support for optimizing medical wastewater treatment facilities and regulating discharge standards. Medical wastewater testing covers multiple categories such as physical and chemical indicators, microbial indicators, toxic and harmful substances, etc. Different indicators adopt targeted testing principles.
Product Details
The composition of medical wastewater is complex, containing pollutants such as pathogenic microorganisms, heavy metals, disinfectants, and drug residues. If not treated to meet standards and discharged, it will seriously threaten the ecological environment and human health. Accurately detecting its water quality is the core link to ensure treatment effectiveness and avoid environmental risks. The following elaborates on the core principles and standardized processes of medical wastewater quality testing.
1、 Core detection principle
Medical wastewater testing covers multiple categories of indicators such as physical and chemical properties, microbial properties, and toxic and harmful substances. Different indicators are tested using targeted testing principles, and the core principles are as follows:
Firstly, physical and chemical index testing. PH value detection is based on the principle of electrode potential. After the glass electrode and reference electrode are inserted into the water sample, the hydrogen ions in the water sample react with the hydration layer on the surface of the glass membrane to generate a potential difference, which is converted into pH value through a pH meter; Suspended solids (SS) use the principle of weight method to intercept suspended particles in water samples through a specific pore size filter membrane, and their content is calculated by drying and weighing; Chemical Oxygen Demand (COD) relies on oxidation-reduction reactions. Under strong acidic conditions, potassium dichromate is used as an oxidant to oxidize reducing substances in water samples. The remaining oxidant is titrated with ammonium sulfite, and the COD value is converted based on the amount of oxidant consumed; Biochemical Oxygen Demand (BOD) is based on the principle of microbial metabolism. Under sealed conditions, microorganisms in water samples decompose organic matter and consume dissolved oxygen. By measuring the difference in dissolved oxygen at different time periods, the five-day Biochemical Oxygen Demand (BOD ₅) is calculated.
Secondly, microbial indicator testing. The detection of pathogenic microorganisms such as total coliforms and fecal coliforms adopts the principle of filter membrane method or multi tube fermentation method. The filter membrane method uses a filter membrane to intercept microorganisms, transfers them to a selective culture medium for cultivation, and determines the number of microorganisms through colony counting; The multi tube fermentation method is based on the fermentation characteristics of microorganisms in a specific culture medium, and the microbial concentration is inferred by the number of positive tubes; The total bacterial count detection adopts the principle of plate colony counting. The water sample is diluted and inoculated into a nutrient medium. After constant temperature cultivation, the number of colonies is calculated to convert the total bacterial count.
Thirdly, detection of toxic and harmful substances. Heavy metals (such as lead, mercury, and cadmium) are analyzed using the principle of atomic absorption spectrophotometry. After the sample is digested, the heavy metal ions absorb the light emitted by the atomizer at a specific wavelength. The absorbance is linearly related to the concentration and can be quantified using a standard curve; Disinfectant residues (such as residual chlorine) are determined using the colorimetric principle. Residual chlorine reacts with specific reagents (such as DPD reagents) to produce colored compounds, whose absorbance is positively correlated with residual chlorine concentration, and can be detected and quantified using a spectrophotometer; Drug residues are often analyzed using high-performance liquid chromatography, based on the differences in distribution coefficients of different substances in the stationary and mobile phases, to achieve component separation and quantitative analysis through detectors.
2、 Standardize the testing process
Medical wastewater testing must strictly comply with standards such as the "Discharge Standards for Water Pollutants in Medical Institutions" (GB 18466-2005). The process includes sampling, sample pretreatment, indicator testing, data processing, and report issuance, as follows:
The first step is sampling preparation and implementation. Before sampling, it is necessary to determine the sampling point (such as the inlet and outlet of the processing facility, key processing units), sampling frequency, and sampling volume according to the testing purpose. Prepare sterile sampling bottles, samplers, insulated boxes, and other equipment, and sterilize the equipment. Strictly follow aseptic operation during sampling, turn on the faucet and drain water for 3-5 minutes to flush the pipeline, then collect water samples, and record basic information such as sampling time, water temperature, pH value, etc. Microbial samples should be filled with sampling bottles to avoid air residue, while physical and chemical samples should be fixed with fixatives (such as nitric acid to fix heavy metal samples) as required. After sampling, they should be promptly refrigerated and transported to the laboratory within 24 hours.
The second step is sample pretreatment. The water sample needs to be homogenized first to remove obvious impurities. For suspended solids, COD and other indicators, if the water sample is turbid, it needs to be filtered through a membrane to remove large particle impurities; Heavy metal detection requires the digestion of water samples, heating with strong acids (such as nitric acid perchloric acid) to destroy organic matter in the sample and convert heavy metals into detectable ionic forms; Microbial samples need to be diluted in a gradient according to the detection method to ensure that the bacterial count after cultivation is within a countable range; For medical wastewater with high oil content, it is necessary to first separate the oil phase through a separating funnel to avoid interfering with the detection results.
The third step is specialized testing of indicators. According to the preset testing plan, each indicator is tested one by one. In the detection of physical and chemical indicators, the pH value is directly measured by a pH meter. Suspended solids are filtered through a filter membrane and dried at 103-105 ℃ before weighing. COD is detected by potassium dichromate digestion combined with titration or rapid digestion spectrophotometry; Microbial indicators need to be operated in a sterile laboratory. The processed samples should be inoculated into a culture medium and placed in a constant temperature incubator (around 37 ℃) for cultivation. After cultivation, counting should be done; Heavy metals and drug residues are detected by precision instruments such as atomic absorption spectrophotometers and high-performance liquid chromatographs, strictly following instrument operating procedures.
Step four, data processing and report issuance. After the detection is completed, the data is sorted and analyzed, blank values are deducted, and the values of each indicator are obtained based on standard curves or calculation formulas. Simultaneously conduct parallel sample error analysis to ensure data accuracy (relative deviation of parallel samples must meet standard requirements). If the data is abnormal, resampling and testing are required to investigate the problem. Finally, a testing report shall be issued in accordance with the standardized format, specifying the core contents such as sampling information, testing methods, testing results of various indicators, and compliance with emission standards, and stamped with the official seal of the testing institution.
In summary, the quality testing of medical wastewater needs to rely on scientific principles and standardized processes, combined with precision instruments and strict quality control, in order to accurately reflect the water quality status and provide reliable data support for optimizing medical wastewater treatment facilities and regulating discharge standards.