Moc1/moc2 mice

Cell name： Moc1/moc2 miceOral squamous cell carcinoma

Cell line:C57BL/6 Cxcr3-/-

Cell source:overseas

Cell identification:STR identification has been passed

Cell morphology:Lymphatic polygonal cell like, adherent+suspended growth

Culture medium:DMEM (containing NaHCO3 1.5g/L) (BasMed-AW-013)+FBS 10%+P/S1% 500ML.

culture conditionsGas phase air, 95%; Carbon dioxide, 5%. Temperature: 37 degrees Celsius, humidity in the incubator is 70% -80%

Cellular background:Oral squamous cell carcinoma (OSCC) is a prominent subset of head and neck cancer, and the main risk factor for OSCC is exposure to carcinogens. This subgroup of head and neck cancer is distinguished from human papillomavirus induced head and neck cancer. However, the prognosis of OSCC has remained stable for decades, leading to an increase in incidence rate and mortality.

Cell usage:For scientific research purposes only

Precautions

1. MOC1 cells have particularly high activity and rapid growth rate. It is not recommended to have too high a passaging density. Instead, it is recommended to choose a thinner layer and spread it again. When the cells reach 80% of their length and are passaged, it is difficult to digest them. They should be placed in an incubator for digestion, which takes about 3-4 minutes before being taken out

2. The adhesion of MOC2 cells is similar to that of conventional cells, but not particularly strong. The doubling cycle is not as fast as MOC1. Treat with conventional digestion methods.

3.Beating is performed on the side of the culture vessel to help cells shed. The beating process takes about 2 minutes to shed most of the cells. If the shedding rate is not very high, it can be returned to the incubator for further digestion for 1-2 minutes, and then taken out again for beating and shedding. After 80-90% of the cells have shed, digestion can be terminated, centrifuged and resuspended, and the bottle can be re placed to disperse evenly.

Shipping at room temperature

upon receiptDisinfect the T25 bottle and place it in the incubator for 2-3 hours. Observe the density and condition, take 2-3 photos, and provide feedback to the sales team. Once the density meets the standard, it can be passaged. The initial passage ratio is 1:2. It is recommended to freeze one whole bottle into a 1ml cryovial and continue passaging the other bottle. Repeat the freezing process for 2-3 bottles before amplification for experimentation to prevent seed breakage in case of unexpected situations.

Dry ice shipment

Conventional Cell Shipping CryovialTwo, one for recovery and the other for backup. In case of unsuccessful recovery, strictly follow the manufacturer's requirements to recover the second one. If there is no successful recovery, please keep a photo of the recovery and notify us immediately.

adherent cells

1. Discard the culture supernatant and use one that does not contain calcium or magnesium ionsWash cells with PBS 1-2 times.

2. join0.25% (w/v) T75 bottles (2-3mL) were placed in a 37 ℃ incubator and digested for 1-2 minutes (difficult to digest cells can be digested for an appropriate extension of time),

Then observe the digestion of cells under a microscope. If most of the cells become round and fall off, quickly take them back to the operating table, lightly tap the culture bottle a few times, and add them3-4 ml of culture medium containing 10% FBS was used to terminate digestion.

３. Gently mix and suck out, thenCentrifuge at 1000RPM for 3-5 minutes, discard the supernatant, add 1-2 mL of culture medium and blow evenly. Divide the cell suspension into new T25 bottles/6cm culture dishes in a ratio of 1:2, and subsequent passages will be conducted in a ratio of 1:2 to 1:5 according to the actual situation.

4. Cell cryopreservationAfter receiving the cells, it is recommended to freeze a batch of cell seeds during the first 3 generations of cultivation for subsequent experiments.

５. Cultivation medium for transportation(Infusion medium) can no longer be used to culture cells. Please use a newly prepared culture medium according to the instructions for cell culture conditions to culture cells.

suspended cells

Cells grown in suspension can be maintained in their growth state by adding culture medium to the culture bottle. Generally, the cell density is maintained at1 × 10 ⁵~1 × 10 ⁶ cells/mL (different cells have different density requirements) can maintain normal cell growth. If necessary, the cell suspension can be collected in a centrifuge tube at 1000rpm and centrifuged for 5 minutes. Discard the supernatant, add 1-2mL of culture medium, resuspend and mix well. Then, divide the cell suspension into new T25 bottles at a ratio of 1:2 and add 6-8ml of new culture medium prepared according to the instructions to maintain the vitality of the cells. Subsequent passages should be carried out at a ratio of 1:2-1:4 according to the actual situation.

biosafety

1. All animal cells are considered to have potential biological hazards and must be operated in a secondary biosafety platform. Please pay attention to protection, and all waste liquids and containers that have come into contact with these cells must be sterilized before disposal.

2. It is recommended to always use protective gloves, clothing, and a face mask when reviving frozen cells. Attention: The cryotube immersed in liquid nitrogen will leak and gradually fill with liquid nitrogen. When thawing, the conversion of liquid nitrogen into gas phase may cause the container to explode or the lid to be blown off with dangerous force, resulting in flying debris and causing personal injury.