Nichiri Mfg high-performance UV spectrophotometer UV8810

Nichiri Mfg high-performance UV spectrophotometer UV8810

1、 Product Introduction (UV8810 Multi Wavelength UV Vis Detector)

UV8810 is a high-performance, multi-purpose UV Vis absorbance detector produced by Nichiri Mfg company in Japan. It is mainly designed for use in high-performance liquid chromatography (HPLC) systems as its core detection component for qualitative and quantitative analysis of separated compounds.

1. Core Features

• Simultaneous detection of multiple wavelengths: This is a significant feature of UV8810. It is usually equipped with a dual beam optical system and multiple photodiodes, which can simultaneously detect samples at multiple wavelengths (commonly 2 or more channels). Users can set a primary wavelength and a secondary wavelength (or multiple secondary wavelengths) in one analysis, greatly improving analysis efficiency and data richness.

• High sensitivity and low noise: The use of high-quality optical components (such as deuterium lamps and photodiodes) and stable electronic design ensures a high signal-to-noise ratio (S/N), which can detect extremely small amounts of samples and meet the requirements of trace analysis.

• Wide wavelength range: covering the typical UV visible region, typically from 190 nm to 700 nm, which means it can detect both compounds with UV absorption (such as aromatic compounds, conjugated systems) and colored substances with absorption in the visible light region.

• Digital signal processing: Built in microprocessor for digital signal control and data processing, improving data accuracy and stability, reducing noise and errors caused by analog signal transmission.

• Easy to operate: usually equipped with a clear display screen and intuitive button or menu interface, making it convenient for users to set wavelengths, adjust ranges, reset, and other operations.

• Powerful output and compatibility: Provides standard analog signal output (such as 0-1V or 1V FS) and digital communication interface (such as RS-232), making it easy to connect with various brands of chromatographic data workstations (CDS) or recorders and integrate into existing HPLC systems.

• Compact and Durable: Japanese instruments are known for their compact design and reliable durability, making them suitable for long-term operation in laboratory environments.

2. Technical specifications (summary)

• Wavelength range: 190-700 nm

• Wavelength setting: 1 nm step, can be accurately set

• Spectral bandwidth: typically 8 nm (fixed bandwidth to ensure good linear range)

• Light source: Long life deuterium lamp (D2 Lamp) and tungsten lamp (W Lamp), the instrument can switch automatically or manually

• Detector: Silicon photodiode

• Flow cell: The standard configuration usually has a 10 mm optical path length and a volume of approximately 8 µ L (other specifications of flow cells can be optionally configured to adapt to different applications)

• Baseline noise:<± 0.35 × 10 ⁻⁵ AU (typical value, extremely low in high sensitivity mode)

• Baseline drift:<± 0.5 × 10 ⁻⁴ AU/hr

• Output interface: Analog output, RS-232C

2、 Product application analysis

As a universal UV Vis detector, UV8810 has an extremely wide range of applications, covering almost all fields that rely on HPLC for separation and analysis.

1. Main application areas

• **Pharmaceuticals * *:

• Determination of active pharmaceutical ingredient (API) content: testing the purity of the main components in the drug.

• Regarding substance (impurity) analysis: monitoring and quantifying degradation products or synthetic by-products in drugs at specific wavelengths.

• Dissolution testing: Online or offline detection of the concentration of drug formulations in the dissolution medium is a key project for quality control of solid formulations.

• Life Sciences and Biochemistry:

• Purification and quantification of proteins, peptides, and nucleic acids (DNA/RNA): Proteins typically have strong absorption at 280 nm (tryptophan, casein), while nucleic acids have absorption at 260 nm. The multi wavelength function can simultaneously monitor 260 nm and 280 nm, and quickly calculate the purity ratio of DNA/RNA (A260/A280).

• Enzyme kinetics research: monitoring the changes in absorbance of reaction products or substrates at specific wavelengths.

• Food and Beverage Industry:

• Additive analysis: detection of preservatives (benzoic acid, sorbic acid), sweeteners (sodium saccharin, aspartame), synthetic pigments, etc.

• Nutritional analysis: determination of the content of vitamins (water-soluble vitamin B group, vitamin C, fat soluble vitamin A, D, E, etc.), sugars, and organic acids.

• Pollution monitoring: such as fungal toxins (aflatoxins, etc.).

• Chemistry and Chemical Engineering:

• Organic synthesis product analysis: monitor reaction progress, determine product purity and yield.

• Quality control of fine chemicals: impurity analysis and content determination of intermediates and finished products.

• Environmental monitoring:

• Environmental pollutants such as polycyclic aromatic hydrocarbons (PAHs), phenols, and pesticide residues are commonly detected by UV detectors after HPLC separation.

2. Utilize advantage analysis

• The value of simultaneous detection of multiple wavelengths:

• Improve selectivity: For complex samples, the optimal absorption wavelength of the target compound and interfering impurities may be different. Simultaneously using two wavelengths (λ 1 for quantifying principal components and λ 2 for monitoring impurities or as a reference wavelength) can significantly improve the selectivity of the method and reduce false positive results.

• Save time and cost: Multiple chromatograms at different wavelengths can be obtained with a single injection, eliminating the need for repeated injections and saving sample, solvent, and analysis time.

• Peak purity identification: By comparing the absorption ratios of a chromatographic peak at different wavelengths, it is possible to preliminarily determine whether the peak is a pure substance or a co eluted mixture.

• The value of high sensitivity:

• Being able to address the challenges of trace analysis, such as in the fields of environmental pollutant detection, drug impurity analysis, etc., regulatory requirements are often extremely low (ppm or even ppb level), and high sensitivity is a prerequisite for meeting these regulatory requirements.

• Reliability and stability:

• Nichiri Mfg, as a professional HPLC component manufacturer, is renowned for its stable and reliable products. This is crucial for quality control (QC) laboratories that require long-term continuous operation, ensuring the repeatability and reproducibility of analysis results.

3. Limitations and precautions

• Not all compounds have UV Vis absorption: For compounds without chromophores (such as some sugars and alcohols), UV detectors cannot detect them and other detectors such as differential refractive index (RID) or evaporative light scattering detector (ELSD) need to be used in conjunction.

• Restricted selection of mobile phase: The mobile phase used must be transparent at the detection wavelength and cannot have strong absorption. For example, when detecting at low wavelengths (<210 nm), many solvents (such as solvents containing benzene rings, acetone) have strong absorption and cannot be used. Methanol, acetonitrile, water, and high-purity buffer salts are usually selected.

• Difference from DAD detector: UV8810 is a multi wavelength detector (MWD) rather than a diode array detector (DAD). DAD can instantly obtain absorption spectra across the entire wavelength range, making it more suitable for identifying unknown substances and evaluating peak purity. MWD (such as UV8810) detects at several discrete wavelengths simultaneously, which is not as comprehensive as DAD in terms of functionality, but usually has lower costs.

summary

The UV8810 multi wavelength UV Vis detector from Nichiri Mfg in Japan is a high-performance, stable, reliable, and widely used HPLC core detection device. The core function of its multi wavelength simultaneous detection gives it significant advantages in method development, selective improvement, and analytical efficiency, making it very suitable for routine quality control, quantitative analysis, and research and development work in fields such as pharmaceuticals, biochemistry, food, chemical engineering, and the environment.

Nichiri Mfg high-performance UV spectrophotometer UV8810