Virus killing effect experiment

Virus killing effect experiment/Report on the effectiveness of virus killing detection

The virus killing effect detection of disinfection products mainly involves selecting representative and live viruses as well as other cell infection techniques, and verifying the virus killing effect of disinfection products' bactericidal factors through various methods. The virus inactivation test plays an important role in demonstrating the ability of disinfection and sterilization factors to inactivate viruses.

After decades of development, Guangdong Huawei Testing Co., Ltd. has become a comprehensive testing institution that integrates product testing, technology research and development, standard setting, academic exchanges, talent cultivation, and science popularization education.
Virus disinfection and sterilization product testing When testing the efficacy of virus killing for disinfection products, virus inactivation analysis will be carried out according to the actual situation of the products, and the efficacy of virus inactivation will be verified.

Core elements of the experiment

1. Virus selectionPriority should be given to selecting representative indicator viruses, including enveloped viruses (such as coronaviruses, influenza virus H1N1) and non enveloped viruses (such as poliovirus, norovirus), with some scenarios using model viruses such as MS2 bacteriophages, balancing safety and cultivation convenience.

2. Experimental carrierChoose the carrier according to the product application scenario, commonly including hard surfaces such as stainless steel and glass, or simulated skin degreasing cotton and simulated air aerosol environment, to ensure it fits the actual user.

3. Concentration and dosage: Set the initial virus concentration (usually ≥ 10 ⁶ PFU/mL), and set the action concentration (such as disinfectant concentration), action time (such as 1min, 5min) and action dose (such as equipment radiation dose, spray amount) according to the product manual.

Main experimental process

1. sample preparationEvenly inoculate the virus suspension onto the experimental vector, dry it, and use it as the sample to be processed; Simultaneously set up a negative control (no viral vector) and a positive control (unsterilized viral vector).

2. Function processingApply the product to the sample to be processed according to the set conditions, and strictly control environmental parameters such as temperature (20-25 ℃) and humidity (40-60% RH) to ensure stable experimental conditions.

3. Virus recovery and detectionRecovery of residual virus on the carrier using a specific eluent, determination of virus titer using plaque assay or half tissue culture infectivity assay (TCID ₅₀), and calculation of kill rate.

4. Result determinationWhen the killing rate is ≥ 99.9% (i.e. log reduction value ≥ 3), it is determined that the product has effective killing effect on the virus; Some high demand scenarios (such as the medical field) require a kill rate of ≥ 99.9999% (log reduction value ≥ 6).

Adhere to core standards

• General disinfection standards: GB/T 38502-2020 "Methods for testing the bactericidal effect of disinfectants in laboratories", EN 14476 "Evaluation of virus killing activity of chemical disinfectants and preservatives".

• Specific scenario standards: Medical device disinfection refers to the GB 18280 series, air disinfection refers to GB/T 18883-2022, and surface disinfection refers to GB 27952-2020.

Virus killing effect experiment/Report on the effectiveness of virus killing detection

The purpose of virus killing efficacy testing is to verify the effectiveness of disinfection products or for daily monitoring. The virus used in virus killing experiments is generally a representative and live virus and its cell infection technology, which evaluates the killing effect of various disinfection factors on the tested virus and verifies the virus killing ability of the disinfection factors.

Virus killing effect test, mainly applicable to disinfection products or daily monitoring. Evaluate the killing effect of various disinfection factors on test viruses using representative and live virus and cell infection techniques. The experiment conducted using this method only verifies an important aspect of the virus inactivation ability of the disinfection factor.

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