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Shanghai Yubo Biotechnology Co., Ltd
Alpha MEM medium
NegotiableUpdate on 04/25
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Overview
Alpha MEM medium: After receiving MEM alpha * medium (containing nucleosides and deoxyribonucleotides), first observe whether the cell bottle is intact and whether there is any leakage or turbidity in the culture medium. If any of the above phenomena occur, please inform us promptly. Carefully read the cell manual to understand cell related information, such as cell morphology, culture medium used, serum ratio, required cytokines, etc.
Product Details
Alpha MEM medium
MEMAlpha * medium(Containing nucleosides and deoxyribonucleotides)Density exceeds90%When the cell state is good, it will be reheated2It needs to be passaged immediately within an hour (the proportion of passaging should depend on different cells), and the state should be stable for stable growth.1Storage: Liquid nitrogen. （2）Transportation: Dry ice. （3）Usage: Can only be used for scientific research. （4）Freezing in liquid nitrogen at the end of the second generation of cultivation. （5）Number of cells per cryovial:>5×105 cells/1ml（6）Not includedHIV-1TheHBVTheHCVMycoplasma, bacteria, yeast, and fungi.
Different cells prefer different environments.
MEMAlpha * medium(Containing nucleosides and deoxyribonucleotides)This is not only about the difference in culture medium, but also about the spatial density of cell growth. Some cells have a larger quantity and better growth status. This usually belongs to cells with slow growth rates. For example, endothelial cells. Some cells, such as macrophages and certain tumor cells, grow better when their number is smaller. Especially macrophages, they grow very quickly and adhere to the wall very quickly, so when passaging, a small amount of cells should be left, so that the cell state will be better. And macrophages prefer to grow in clusters, and there is space between clusters.MEMAlpha * medium(Containing nucleosides and deoxyribonucleotides)If it grows into a connected whole, the cell morphology will basically deteriorate, and there will be more aging, which is not good for the later experimental results. So when nurturing cells, we should explore the density of the growth space that the cell likes.
How to choose a culture medium?
Alpha MEM medium：There are no fixed culture conditions for cultivating a certain type of cell. inMEMCells cultured in the medium are likely toDMEMorM199It is also easy to grow in the middle. In short*MEMPerforming adherent cell cultureRPMI-1640For suspension cell culture and serum-free culture for various purposes *AIM VCulture medium（SFM）. When starting a new cell culture.
