Cytiva TALON Superflow Filler

Cytiva TALON Superflow Filler28957502 28957499

TALON ® Superflow ™ It is a cobalt based IMAC filler that provides a high-purity nickel filler substitute for purifying amino acid labeled proteins.

Due to its small size and minimal interference with the function, activity, or structure of the target protein, the histone suan tag is widely used. Fixed metal ion affinity chromatography (IMAC) is a method for purifying histonessuanCommon methods for labeling proteins. IMAC chromatography media selectively loaded with covalent metal ions (such as nickel or cobalt) can trap ammoniasuanLabel protein, and under denaturing conditions, can purify insoluble histones from inclusion bodiessuanLabel protein. Successful IMAC purification can obtain the target protein with high purity, high yield, and high activity.

Because many proteins have inherent histonessuanAnd/or cysteinesuanAmino acid residues and other non-specific proteins can bind to the IMAC filling medium together with the target protein. In this case, it is necessary to frequently optimize the binding, washing, and elution conditions by changing the concentration of imidazole in the solution. Increasing the concentration of imidazole in the binding and washing buffer typically reduces non-specific binding, while lower concentrations lead to stronger affinity interactions. Finding the right balance is crucial.

Cytiva TALON Superflow Filler28957502 28957499 Order information:

28957499/28-9574-99：TALON Superflow, 10 mL

28957502/28-9575-02：TALON Superflow, 50 mL

29048565/29-0485-65: HiTrap TALON crude,1*1ml

28953766/28-9537-66: HiTrap TALON crude,5*1ml

28953805/28-9538-05: HiTrap TALON crude,100*1ml

28953767/28-9537-67: HiTrap TALON crude,5*5ml

28953809/28-9538-09: HiTrap TALON crude,100*5ml