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Shanghai Yubo Biotechnology Co., Ltd
DMEM culture medium price
NegotiableUpdate on 04/25
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Overview
The price of DMEM culture medium. After receiving the high sugar DMEM removal medium for "black glue worms", first observe whether the cell bottle is intact and whether there is any leakage or turbidity in the culture medium. If any of the above phenomena occur, please inform us in a timely manner. Carefully read the cell manual to understand cell related information, such as cell morphology, culture medium used, serum ratio, required cytokines, etc.
Product Details
DMEM culture medium price
Cleaning medium for "black glue insect" （DMEMHigh sugar)To ensure the normal growth of cells during transportation, please consult us for pricing, quotation, and delivery time.
Characteristic: The number of cell generations is2-3Left and right.
Cell use: Provide stable cell lines for scientific research.
Cell purity:92％
Cell viability:88％（ViabilitybyTrypanBlueExclusion）.
Cell detection: Cells do not containHIV-1TheHBVTheHCVMycoplasma, bacteria, yeast, and fungi.
Storage method: Liquid nitrogen (frozen) or revival culture.
Specifications:500000cells/bottle
Cleaning medium for "black glue insect" （DMEMHigh sugar)experimental results
1.Regularly observe the formation of colonies during the cell culture process.
2.Counting under the microscope is greater than50Calculate the colony formation rate based on the number of cell clones and the following formula:
Colony formation rate（%）=(Number of colonies)/Number of cells inoculated) ×100
Cleaning medium for "black glue insect" （DMEMHigh sugar)Precautions
1.Agar is unstable to heat and acid, and if repeatedly heated, it is prone to degradation, toxicity, and a decrease in agar hardness. Agar high-pressure sterilization( 10pound 15minute)Then pack according to a single dose.
2.Cell dispersion in cell suspension>95%.
3.When cultivating with soft agar, be careful not to exceed the temperature when mixing the agar with cells 40℃ To avoid burning the cells.
4.The density of inoculated cells should not be too high.
5.”Cleaning medium for black gum worms （DMEMHigh sugar)Under low-density conditions, the survival rate significantly decreases, and the clone formation rate of infinite cell lines and tumor cell lines is generally around10%The above. But the first generation cultured cells and limited cell lines are only0.5~5%,Even zero. To improve the colony formation rate, it is necessary to add substances such as insulin and * that promote cell colony formation to the culture medium.
DMEM culture medium price
