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Shanghai Boke Biotechnology Co., Ltd
IL-1 β ELISA kit
NegotiableUpdate on 02/19
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Overview
IL-1 β ELISA kit reading plate: 1. The negative control has a very light color, and visual colorimetry can generally be used for qualitative determination. If the results are measured using an ELISA reader, the accuracy depends on the flatness and transparency of the ELISA plate bottom, the quality of the ELISA reader, and the algorithm of the software.
Product Details
IL-1 β ELISA kitMeasurement steps:
1. Sample addition
1. Except for the bedding, samples need to be added at a 45 degree angle
2. The sample volume should be accurate
3. Sample should be added to the bottom of the pipe, not to the wall of the pipe
4. No bubbles should be generated during sample addition
2. Warm bath
After adding the specimen and the conjugate, they should be immediately placed in a water bath at the specified reaction temperature.
2. The ELISA plates should not be stacked together.
To avoid evaporation, the board should be covered or placed flat in a metal wet box with wet gauze at the bottom.
After adding the substrate, the reaction time and temperature are usually not strictly required. If the room temperature is above 20 ℃, the ELISA plate can be placed in the dark on the experimental bench for occasional observation. When the control tube develops color appropriately, the enzyme reaction can be terminated.
3. Washing
1. Washing is not a reaction step in the ELISA process, but it is the key to determining the success or failure of the experiment.
2. The purpose is to wash away substances in the reaction solution that have not bound to the solid-phase antigen or antibody, as well as interfering substances that have non-specific adsorption on the solid-phase carrier during the reaction process.
4. Reading board
1. The color of the negative control is extremely light, and visual colorimetry can generally be used in qualitative testing.
If the results are measured using an ELISA reader, the accuracy depends on the flatness and transparency of the ELISA plate bottom, the quality of the ELISA reader, and the algorithm of the software.
1. Sample addition
1. Except for the bedding, samples need to be added at a 45 degree angle
2. The sample volume should be accurate
3. Sample should be added to the bottom of the pipe, not to the wall of the pipe
4. No bubbles should be generated during sample addition
2. Warm bath
After adding the specimen and the conjugate, they should be immediately placed in a water bath at the specified reaction temperature.
2. The ELISA plates should not be stacked together.
To avoid evaporation, the board should be covered or placed flat in a metal wet box with wet gauze at the bottom.
After adding the substrate, the reaction time and temperature are usually not strictly required. If the room temperature is above 20 ℃, the ELISA plate can be placed in the dark on the experimental bench for occasional observation. When the control tube develops color appropriately, the enzyme reaction can be terminated.
3. Washing
1. Washing is not a reaction step in the ELISA process, but it is the key to determining the success or failure of the experiment.
2. The purpose is to wash away substances in the reaction solution that have not bound to the solid-phase antigen or antibody, as well as interfering substances that have non-specific adsorption on the solid-phase carrier during the reaction process.
4. Reading board
1. The color of the negative control is extremely light, and visual colorimetry can generally be used in qualitative testing.
If the results are measured using an ELISA reader, the accuracy depends on the flatness and transparency of the ELISA plate bottom, the quality of the ELISA reader, and the algorithm of the software.
Experimental standard image:
40 ng/L， Standard 5, add 150 μ l of original standard to 150 μ l of standard dilution solution
20 ng/L， Standard No. 4, add 150 μ l of standard No. 5 to a dilution of 150 μ l of standard solution
10 ng/L， Add 150 μ l of standard dilution solution to 150 μ l of standard No. 3 and standard No. 4
5 ng/L， Standard No. 2, add 150 μ l of standard No. 3 to a dilution of 150 μ l of standard solution
2.5 ng/L， Add 150 μ l of standard dilution solution to 150 μ l of standard 1 and 150 μ l of standard 2
One of our main products is strictly produced according to relevant standards and undergoes rigorous and repeated testing. We guarantee the quality of our products with a rigorous attitude, ensuring the smooth progress of your experiments. The product has a short supply cycle, timely delivery, and our company also provides complete pre-sales and after-sales services
IL-1 β ELISA kitReagent preparation:
1. Enzyme linked plate: one piece (96 well or 48 well).
2. Standard: 2 bottles (freeze-dried).
3. Sample Diluent: 1 × 20ml/bottle.
4. Biotin antibody diluent: 1 × 10ml/bottle.
5. HRP avidin diluent labeled with horseradish peroxidase: 1 × 10ml/bottle.
6. Biotin antibody labeling: 1 × 120 μ l/bottle (1:100)
7. Horseradish peroxidase labeled avidin (HRP avidin): 1 × 120 μ l/bottle (1:100)
8. TMB Substrate: 1 × 10ml/bottle.
9. Wash Buffer: 1 × 20ml/bottle, diluted 25 times with distilled water per bottle during use.
10. Stop Solution: 1 × 10ml/bottle (2N H2SO4).
