IL-18 Mouse IL-18 ELISA Detection Kit

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We can tailor testing kits according to your application needs, such as special requirements for detection range, species, and sensitivity, to effectively control the cost of testing reagents. And free proxy testing can be provided.

parameter

Storage conditions:Low temperature storage at 2-8 ℃

Shelf life:Within 6 months, all reagent kits will be provided with new batches.

Ingredients of the reagent kit: enzyme-linked immunosorbent assay (ELISA) plate, reagents, standards, etc.

Select specifications:

Product Specifications48T/96T

48T can produce 37 samples

96T can produce 85 samples

Main components: Enzyme linked immunosorbent assay (ELISA) plateReagents, standards, etc.

Species of reagent kit: potatoes, deer, sheep, chickens, ducks, fish, humans, rats, mice, guinea pigs, hamsters, nude mice, rabbits, pigs, dogs, monkeys, horses, cows and other animals and plants.

Purpose of testing: Used to determine samples such as serum, plasma, and related fluids. For example, suitable for detecting specimens including serum, plasma, urine, pleural and peritoneal fluid, lavage fluid, cerebrospinal fluid, cell culture supernatant, tissue homogenate, etc..

Reagent preparation:

1. Before use, slowly equilibrate all reagents and specimens to room temperature (18-25oC). Reagents should not dissolve directly at 37oC.

2. Standard (freeze-dried): Add 1mL of standard diluent to each bottle of standard, cover and let it stand at room temperature for about 10 minutes, while repeatedly reversing/rubbing to aid dissolution. The concentration is 2000pg/mL. Prepare 7 EP tubes for diluting standard samples, add 150 μ L of standard dilution solution to each EP tube, and dilute them in different ratios as shown in the figure. The standard dilution solution (0pg/mL) is directly used as a blank well. To ensure the validity of the experimental results, please use a new standard solution for each experiment.

3. Concentrated washing solution: Dilute 20mL of concentrated washing solution to 600mL with 580mL of distilled water or deionized water, and dilute 30 times.

Preparation before sample experiment:

ELISA kit liquid samples: including serum, plasma, urine, pleural and peritoneal fluid, cerebrospinal fluid, cell culture supernatant, etc.

（1) Serum

Natural coagulation of blood at room temperatureAfter 10-20 minutes, centrifuge for about 20 minutes (2000-3000 revolutions per minute). Carefully collect the supernatant. If precipitation forms during storage, it should be centrifuged again.

（2) Plasma:

Selection should be based on the requirements of the specimenEDTA、 Sodium citrate or heparin is used as an anticoagulant. After mixing for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant. If precipitation forms during storage, it should be centrifuged again.

（3) Urine:

Collect with sterile tubes. centrifugationAbout 20 minutes (2000-3000 revolutions per minute). Carefully collect the supernatant. If precipitation forms during storage, it should be centrifuged again. Follow this procedure for pleural effusion, ascites, and cerebrospinal fluid.

（4) Cell culture supernatant:

When detecting secretory components, collect them using sterile tubes. centrifugationAbout 20 minutes (2000-3000 revolutions per minute). Carefully collect the supernatant.

（5) Cultivate cells

When detecting the components inside cells, useDilute the cell suspension with PBS (pH 7.2-7.4) to achieve a cell concentration of around 1 million/ml. By repeatedly freezing and thawing or adding tissue protein extraction reagents, cells are destroyed and intracellular components are released. Centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant. If precipitation forms during storage, it should be centrifuged again.

（6) Organizational specimen

After cutting the specimen, weigh it. Add a certain amountPBS，PH7.4。 Quickly freeze and store in liquid nitrogen for future use. The specimen remains at a temperature of 2-8 ℃ even after melting. Add a certain amount of PBS (pH 7.4) or tissue protein extraction reagent, and homogenize the specimen manually or with a homogenizer. Centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant. After packaging, one portion is to be tested, and the rest is to be frozen for future use.

Notes:

1. The reagent kit should be used within its expiration date, please do not use expired reagents.

When the reagent kit is not in use, it should be stored in a refrigerator at 2-8 ℃. Standard samples that have been dissolved but not used up should be discarded.

Before using the reagent kit, please allow it to recover at room temperature for 30 minutes and thoroughly mix all the components and prepared samples in the kit.

4. In the experiment, it is recommended to perform double well testing on the standard and sample, and the order of adding reagents should be consistent.

To avoid cross contamination, please use disposable test tubes, gun heads, sealing films, and clean plastic containers in the experiment.

6. The volume of concentrated antibody and enzyme complexes is relatively small, and trace amounts of liquid may stick to the tube wall and bottle cap during transportation. Before use, please centrifuge (5-10 seconds is sufficient) to concentrate the liquid on the tube wall at the bottom. When using, please use a pipette to carefully blow and tap several times.

7. Except for the concentrated washing solution and termination solution in the reagent kit that can be used interchangeably, please do not use reagents from other sources in the reagent kit to replace a single component in this kit.

To ensure accurate results, a standard curve must be drawn for each test.

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