RNA Nucleic Acid Extraction and Purification Kit

The * nature of technology:

（1） High sensitivity

Although the sensitivity of enzyme activity regulated ELISA method is currently not ideal, the detection sensitivity in enzyme activity amplified ELISA is much higher than that of RIA. According to the law of mass action. The amount of immune complexes formed by immune reactions is directly proportional to the concentration of reactants. It is speculated that the number of molecules to be detected is 1. Given that one molar concentration contains 6.02 × 1023 molecules, it is theoretically speculated that the lowest detection limit of the enzyme activity amplification Elisa method can reach 1.7 × 10-24mol/L. Although in practical applications, due to factors such as reaction conditions, reagent purity, and instrument accuracy, it is often not possible to achieve this level (greater than 104 molecules), it indicates that Elisa has great potential for improving sensitivity.

（2） Strong specificity

From the perspective of immune response, the specificity of Elisa and RIA should be. However, in the ELISA method, the specificity of the reaction between the enzyme that acts as the detection indicator and its substrate increases the specificity of the method.

（3） Low requirements for instruments and equipment, low measurement cost

Elisa assay can be performed in ordinary laboratories, and commonly used instruments and equipment include sample dispensers, incubators, enzyme-linked immunosorbent assay readers, etc. Based on current prices, the price of an enzyme-linked immunosorbent assay (ELISA) reader is only 1/6 to 1/4 of that of a liquid scintillation analyzer, so the cost of measuring each sample is less than 1/10 to 1/16 of that of PIA. Some qualitative Elisa methods can also be performed in the field, making the operation very convenient.

（4） The method is fast and simple

When using the homogeneous enzyme immunoassay method, all reaction reagents are carried out in the same system without any separation steps. The operation is very simple and fast, and the results can be obtained in a few minutes. Some qualitative Elisa kits, such as those produced abroad for identifying estrus and diagnosing pregnancy in cows, can complete a measurement in just a few minutes.

（5） Reagent storage time is relatively long

Enzymes and enzyme markers used as detection indicators are relatively stable under low temperature or dry conditions and can be stored for six months to several years.

（6） High degree of automation

Elisa, due to the absence of radioactive isotope contamination, can automate all steps except for adding the test sample, which requires manual operation. Under these automated conditions, an average of over 2000 samples can be tested per person per day.

（7） Multiple types of methods

ELISA technology can fully utilize the advantages of monoclonal antibodies and utilize the effects of certain non immune reactive reagents (such as SPA, lectins, etc.), developing into many new methods. In addition, the development of Elisa technology can also be approached from two aspects: enzymes and their substrates. This is because: * There are far more types of enzymes used in ELISA technology than radioactive isotopes that can be used as indicators for RIA detection. There are various enzymes in the biological world, and there is hope to develop many types of enzymes for Elisa and establish corresponding ELISA methods. On the contrary, the chemical elements in nature are limited, and the types of radioactive isotopes are even more limited. *Due to the diversity of enzymes, there are also various substrates for enzyme action, and the corresponding types of chromogenic sources or hydrogen donors also have great potential for development and advancement.