-
E-mail
sale1@shybsw.net
-
Phone
18321282235
-
Address
Room 806, 8th Floor, Longzhimeng Building, 6088 Humin Road, Shanghai
Product Categories
Shanghai Yubo Biotechnology Co., Ltd
PK-59 cells
NegotiableUpdate on 04/25
- Model
- Nature of the Manufacturer
- Producers
- Product Category
- Place of Origin
Overview
PK-59 cells were extracted and purified from induced cells of mouse X, and immediately frozen. This cell was validated by vWF/Factor VIII and CD31 (P-CAM) immunofluorescence staining and tested to be free of HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. All cells undergo strict cell quality testing and identification before being stored to ensure that they reach every user's hands
Product Details
Product Name:PK-59 cells, Mouse X Mouse
Cell growth: adherent growth or suspended growth
Cell morphology: epithelioid, polymorphic cell like, and other forms
Cell purity: 93%
活力： 87％（Viability by Trypan Blue Exclusion）
Cell detection: Cells do not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi
Cell transport: Dry ice transport (1 Vial) or live cell transport (T-25 flakes)
Cell use: only for scientific research, not for clinical diagnosis and treatment
PK-59 cells, Mouse X MouseThe separation method is as follows:
① Overnight cold digestion: Wash the obtained tissue three times with Hanks' solution, cut it into fragments of about 4 millimeters in size, wash 2-3 times with Hanks' solution to remove blood cells and adipose tissue, add 0.25% *, shake well, and let it sit overnight at 4 ℃. The next day, wash again with Hanks' solution, discard the supernatant, wash 2-3 times in total, then add a small amount of nutrient solution, blow and disperse, count the cells, and culture them in appropriate concentration bottles.
② There are three types of multiple extraction and digestion methods:
1Multiple extractions were performed by hot digestion, and the cut cell blocks were added to a 0.25% * 37 ℃ water bath for digestion for 15-20 minutes,Then, after washing, the cells were dispersed in nutrient solution to form a cell suspension, which was cultured in appropriate concentration bottles. The remaining undigested tissue was then digested and extracted using the above method.
2The extraction method for multiple cold digestion is the same as above, except that the digestion temperature is 4 ℃.
3First, hot digestion followed by cold digestion. The tissue blocks were first digested with * at 37 ℃ for 20 minutes, washed, and then dispersed with nutrient solution to make a suspension. The remaining undigested small group of tissue blocks were washed and then used with * at 4 ℃ overnight. The cells were extracted the next day, dispersed into a suspension, and cultured in separate bottles.
Spot supply, more related products are as follows:Kl1854 hPECAM ELISA Human PECAM-1/CD31 ELISA Kit 96 tests
