TE-6 cells

NegotiableUpdate on 04/25

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Nature of the Manufacturer: Producers

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Overview

The proliferation ability of TE-6 cells (human esophageal cancer cells) depends on comprehensive factors such as cell sampling, culture techniques, and culture conditions. Please follow the correct cultivation method for thawing and passage to ensure that the cells have good proliferation ability and facilitate the smooth progress of your subsequent research.

Product Details

Cell name:TE-6 cells

Human esophageal cancer cells

Cultivation conditions:RPMI-1640 +10% FBS

Form:wall sticking

TE-6(Human esophageal cancer cells)The separation method is as follows:

① Overnight cold digestion: Wash the obtained tissue three times with Hanks' solution, cut it into fragments of about 4 millimeters in size, wash 2-3 times with Hanks' solution to remove blood cells and adipose tissue, add 0.25% *, shake well, and let it sit overnight at 4 ℃. The next day, wash again with Hanks' solution, discard the supernatant, wash 2-3 times in total, then add a small amount of nutrient solution, blow and disperse, count the cells, and culture them in appropriate concentration bottles.

② There are three types of multiple extraction and digestion methods:

1Multiple extractions were performed by hot digestion, and the cut cell blocks were added to a 0.25% * 37 ℃ water bath for digestion for 15-20 minutes,TE-6(Human esophageal cancer cells)Then, after washing, the cells were dispersed in nutrient solution to form a cell suspension, which was cultured in appropriate concentration bottles. The remaining undigested tissue was then digested and extracted using the above method.

2The extraction method for multiple cold digestion is the same as above, except that the digestion temperature is 4 ℃.

3First, hot digestion followed by cold digestion. The tissue blocks were first digested with * at 37 ℃ for 20 minutes, washed, and then dispersed with nutrient solution to make a suspension. The remaining undigested small group of tissue blocks were washed and then used with * at 4 ℃ overnight. The cells were extracted the next day, dispersed into a suspension, and cultured in separate bottles.

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