TK10 cells

Product Name:TK10 cells

Specifications:25T

Cultivation conditions:DMEM(High sugar)+10%FBS

Passage method:1:3Passage,3-4Change the fluid once every day

Freezing conditions:90%FBS+10%DMSOAfter receiving the cells, observe the overall growth of the cells under an inverted microscope:

if【TK10 cells】Not fully grown, use75%After disinfecting the entire bottle with alcohol spray, place it in the super sterilization table and perform strict aseptic operation. Open the cell culture bottle and leave it10mlContinue to cultivate with the culture medium.

If the cell is already full (up to)80-90%）. It can be passaged, and the specific steps are as follows:

1Discard the culture medium and usePBSwash1-2Next time.

2Add to the bottle1.0-2.0ml*Liquid, observe the digestion of cells under an inverted microscope. If most of the cells become round, quickly take them back to the operating table, aspirate *, and add * containing6mlcontain10%Gently blow and beat the cells in the serum culture medium.

3Add an equal amount of culture medium, gently blow and mix well, then aspirate half and transfer to new culture

4Passage ratio:1:2-1:3

If the growth is slow:

1.Compare the differences in glucose, amino acids, and other components in different culture medium formulations with changes in culture medium or serum; Compare the differences between new and old batches of serum through growth experiments; Increase the initial seeding density of cells; Gradually adapt cells to the new culture medium.

2.Essential growth promoting ingredients (such asL-*Depletion, deficiency, or decomposition of growth factors (or growth factors), removal of the original culture medium, and addition of fresh culture medium; Add growth promoting ingredients (such asL-*).

3.Cultivate cells with mild bacterial or fungal contamination without adding antibiotics. If the cells are contaminated, they should be sterilized and discarded.

4.Improper time storage of serum should be addressed-5℃ to-20Storage at ℃; The culture medium should be2℃~8Store in the dark at ℃; Try to minimize the exposure time of serum and culture medium to light.

5.Increase the seeding density of live cells when the initial seeding density is low.

6.Cellular aging involves discarding aging cells and using cells with fewer generations.

7.Isolate cells contaminated with mycoplasma and test for mycoplasma infection; Clean the ventilated kitchen and incubator, and discard them after sterilization if the cells are contaminated.

List of other cell lines:Lewis lung cancer cell brand ATCC specification 25 milliliters per strain Delivery time 1-2 weeks